Description

5-Fluorouracil-15N2 is a stable isotope-labeled analog of the widely used antimetabolite and chemotherapeutic agent, 5-fluorouracil. This compound, with CAS number 68941-95-7, is an essential tool for advanced pharmaceutical research and development, particularly in pharmacokinetic and metabolic studies. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and contract research organization (CRO) sectors who require high-purity, isotopically labeled internal standards for mass spectrometry.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference in liquid chromatography-tandem mass spectrometry for the accurate bioanalysis of 5-fluorouracil in biological matrices.
• Drug Metabolism and Pharmacokinetics (DMPK): Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) pathways in preclinical and clinical research.
• Isotope Dilution Mass Spectrometry (IDMS): Used as a spike in IDMS methods to achieve high-accuracy quantification, minimizing matrix effects and improving analytical reliability.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of metabolites or degradation products during drug development and stability testing.
• Clinical Research Assays: Supports the development of robust diagnostic assays for therapeutic drug monitoring in oncology patients.
• Biochemical Pathway Tracing: Facilitates studies on the mechanism of action and cellular uptake of fluoropyrimidine-based therapies.

Basic Information

Product Name	5-Fluorouracil-15N2
CAS No.	68941-95-7
Molecular Formula	C4H3F15N2O2
Molecular Weight	132.04 g/mol (for 15N2 labeled form)
Synonyms	5-FU-15N2; 5-Fluoro-1H-pyrimidine-2,4-dione-15N2; 5-Fluorouracil (15N2); 5-FU (15N2); 5-Fluoropyrimidine-2,4(1H,3H)-dione-15N2; 5-Fluoro-2,4(1H,3H)-pyrimidinedione-15N2; NSC-19893-15N2; Adrucil-15N2 (labeled analog)
EINECS	Contact for details

Quality Control

Our 5-Fluorouracil-15N2 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, chemical purity, and isotopic enrichment. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment away from direct light exposure to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % 15N
Chemical Purity (by HPLC)	≥ 99.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.