Description

Dibromobimane CAS NO 68654-25-1 is a highly reactive, bifunctional thiol-specific alkylating agent widely used as a fluorescent labeling reagent. Its core value lies in its ability to form stable, fluorescent adducts with thiol groups, enabling sensitive detection and quantification in complex matrices. This compound is essential for researchers and manufacturers in the life sciences, particularly for protein chemistry, proteomics, and diagnostic assay development.

Application

• Protein Thiol Group Labeling: For fluorescence detection, quantification, and structural studies of cysteine residues in proteins and peptides.
• Proteomics Research: Used in 2D fluorescence difference gel electrophoresis (2D-DIGE) to label protein samples for comparative analysis.
• Diagnostic Assay Development: Serves as a key reagent in developing fluorescent assays for detecting biological thiols like glutathione (GSH) in clinical and research settings.
• Cell Biology Studies: Employed to label intracellular thiols for imaging and flow cytometry, providing insights into redox states and oxidative stress.
• Pharmaceutical Analysis: Utilized in the analysis and quality control of drug substances and products containing thiol or disulfide bonds.
• Bioconjugation: Acts as a crosslinker or labeling agent for attaching fluorescent tags to antibodies, enzymes, and other biomolecules.

Basic Information

Product Name	Dibromobimane
CAS No.	68654-25-1
Molecular Formula	C10H10Br2N2O2
Molecular Weight	358.01 g/mol
Synonyms	mBBr; Monobromobimane; 1,3-Dibromomethyl-3,5,5-trimethyl-2,4-imidazolidinedione; Bimane, 1,3-dibromo-; Thiolite™ (trade name variant); Thiol-specific fluorescent probe; DBB; Dibromomethylbimane
EINECS	Contact for details

Quality Control

Our Dibromobimane is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the container to reach ambient temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.