Description

Affi-Gel 501 is a specialized activated affinity chromatography medium designed for the covalent immobilization of thiol-containing ligands. This product is critical for researchers and process developers requiring high-performance, stable ligand coupling for the purification of biomolecules. It is essential for downstream processing in the biopharmaceutical and life sciences industries, enabling the selective capture of target proteins, antibodies, and other biological entities.

Application

• Purification of antibodies, enzymes, and recombinant proteins via thiol-coupling strategies.
• Immobilization of peptides, oligonucleotides, or small molecules containing free sulfhydryl groups for affinity chromatography.
• Development of custom biosensors and diagnostic assay components requiring stable surface conjugation.
• Preparation of solid-phase catalysts for bioconjugation and chemical synthesis applications.
• Research-scale and process-scale purification in academic, biotech, and pharmaceutical R&D.
• Creation of specialized chromatography columns for the isolation of specific biomolecular targets.

Basic Information

Product Name	Affi-Gel 501
CAS No.	68652-24-4
Molecular Formula	Contact for details
Molecular Weight	N/A (Chromatography resin)
Synonyms	Affi-Gel 501 Activated Affinity Gel; Activated CH-Sepharose 4B; 2-Pyridyldisulfide-activated agarose; Activated Thiol Sepharose 4B; Pyridyl disulfide-activated cross-linked agarose; Reactive gel for thiol coupling; Thiopropyl Sepharose 6B activation intermediate; Affinity chromatography medium, activated for SH groups
EINECS	Contact for details

Quality Control

Our Affi-Gel 501 is manufactured under a quality management system and undergoes rigorous batch testing to ensure performance consistency for critical separations. Key parameters such as activation level, ligand density, bead size distribution, and flow properties are characterized. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. Do not freeze. Keep away from strong bases and organic solvents. The product is supplied as a suspension; ensure the storage container remains sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked 4% or 6% beaded agarose
Activation Group	2-Pyridyldisulfide
Ligand Density	10 - 30 µmol/mL gel (typical)
Bead Size	45 - 165 µm (wet)
Exclusion Limit	> 1x10⁷ Daltons
Maximum Flow Rate	150 - 300 cm/hr (varies with column packing)
pH Stability	2 - 11 (long term), 3 - 9 (working)
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.