Description

Ubiquitin Hexadecapeptide CAS NO 67829-15-6 is a synthetic peptide fragment corresponding to a specific 16-amino acid sequence of ubiquitin, a highly conserved regulatory protein found in all eukaryotic cells. This well-defined peptide is a critical tool for biochemical research, enabling the study of ubiquitin's structure, function, and its role in the vital ubiquitin-proteasome pathway for protein degradation. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on proteomics, signal transduction, and drug discovery for conditions like cancer and neurodegenerative diseases.

Application

• Biochemical Research Reagent: Used as a standard or substrate in enzymatic assays to study ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2), and ubiquitin ligases (E3).
• Antigen for Antibody Production: Serves as an immunogen to generate polyclonal or monoclonal antibodies specific to ubiquitin or its epitopes for use in Western blotting, ELISA, and immunohistochemistry.
• Proteomics and Mass Spectrometry: Employed as a reference peptide for the calibration of mass spectrometers and in peptide mapping studies to understand protein modifications.
• Inhibition and Binding Studies: Utilized in competitive binding assays to investigate the interaction between ubiquitin and its binding partners, such as deubiquitinating enzymes (DUBs) or ubiquitin-binding domains (UBDs).
• Cell Signaling Pathway Analysis: Applied in research to dissect the role of ubiquitination in critical cellular processes including cell cycle regulation, DNA repair, and immune response.
• Pharmaceutical Development: Acts as a key intermediate or reference compound in the discovery and development of therapeutics targeting the ubiquitin-proteasome system.

Basic Information

Product Name	Ubiquitin Hexadecapeptide
CAS No.	67829-15-6
Molecular Formula	C78H131N23O25S
Molecular Weight	1835.06 g/mol
Synonyms	Ubiquitin (1-16); Ubiquitin Peptide (1-16); Ubiquitin 1-16 Fragment; Ubiquitin Hexadecapeptide (1-16); MQIFVKTLTGKTITLE; Ubiquitin N-terminal Hexadecapeptide; Ubiquitin Residues 1-16; Ubiquitin-Derived Peptide
EINECS	Contact for details

Quality Control

Our Ubiquitin Hexadecapeptide is manufactured under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including reverse-phase HPLC for purity assessment, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). Certificates of Analysis (COA) detailing purity, peptide content, and solvent residues are provided to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, consider lyophilized powder under inert atmosphere. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. Keep away from strong acids and bases.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by AAA)
Single Impurity (HPLC)	≤ 1.0%
Amino Acid Composition	Within ±10% of theoretical
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.