Description

Cholesterol Dehydrogenase CAS NO 67775-34-2 is a highly specific oxidoreductase enzyme that catalyzes the conversion of cholesterol to cholest-4-en-3-one. This enzymatic activity is critical for precise biochemical analysis and diagnostic applications, ensuring accurate and reliable results. It is an essential reagent for manufacturers and research institutions in the clinical diagnostics, pharmaceutical research, and food safety testing sectors, where quantitative cholesterol measurement is paramount.

Application

• Clinical Diagnostic Kits: Core component in enzymatic colorimetric and fluorometric assays for the quantitative determination of total and free cholesterol in human serum, plasma, and other biological fluids.
• Pharmaceutical Research & Development: Used in enzymatic studies, inhibitor screening, and metabolic pathway analysis related to cholesterol biosynthesis and regulation.
• Food & Beverage Analysis: Employed in enzymatic methods to quantify cholesterol content in dairy products, meats, edible oils, and other foodstuffs for nutritional labeling and quality control.
• Biochemical Reagent Manufacturing: Sourced as a key ingredient for producing high-purity reagent kits and standards used in laboratory analysis.
• Academic & Industrial Research: Utilized in studies of lipid metabolism, membrane biophysics, and the development of novel biosensor technologies.

Basic Information

Product Name	Cholesterol Dehydrogenase
CAS No.	67775-34-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cholesterol Oxidase (microbial); 3β-Hydroxysteroid:NAD(P)+ Oxidoreductase; Cholest-5-en-3β-ol:NAD(P)+ 3-oxidoreductase; NAD(P)-dependent cholesterol dehydrogenase; CHO; COD; Sterol dehydrogenase
EINECS	Contact for details

Quality Control

Our Cholesterol Dehydrogenase is manufactured and tested under a rigorous quality management system. Each batch is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive diagnostic and research applications. Certificates of Analysis (COA) documenting key parameters such as activity units, protein content, and purity are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ XX U/mg protein (Contact for details)
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Contact for details
Stability	Retains ≥ 90% activity after XX months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.