Description

Blue Dextran-Sepharose is a specialized affinity chromatography medium designed for the separation and analysis of macromolecules. This product is essential for researchers and process developers requiring a high-capacity, cross-linked matrix for gel filtration calibration, void volume determination, and the purification of specific proteins or enzymes. It is a critical tool for biotechnology, pharmaceutical development, and academic research laboratories focused on downstream processing and molecular characterization.

Application

• Calibration standard for determining the void volume (V0) in gel filtration chromatography columns.
• Tool for studying the hydrodynamic properties and molecular size exclusion limits of various chromatographic media.
• Affinity matrix for the purification of carbohydrate-binding proteins, such as certain lectins and enzymes.
• Model substrate in bioprocess development for testing and scaling up chromatographic separation protocols.
• Educational and research tool in biochemistry and molecular biology for demonstrating principles of size-exclusion chromatography.
• Preparative-scale separation of high molecular weight blue-colored complexes or conjugated biomolecules.

Basic Information

Product Name	Blue Dextran-Sepharose
CAS No.	67701-59-1
Molecular Formula	N/A (Chromatographic Medium)
Molecular Weight	~2,000,000 (for Blue Dextran component)
Synonyms	Blue Dextran 2000-Sepharose; Sepharose 4B cross-linked with Blue Dextran 2000; Blue Dextran-Sepharose 4B; Cibacron Blue F3G-A–Sepharose (related); Dextran Blue-Sepharose CL-6B; Affi-Gel Blue (analogous product); Blue Sepharose CL-6B; Chromatography Medium Blue Dextran
EINECS	Contact for details

Quality Control

Our Blue Dextran-Sepharose is manufactured under controlled conditions to ensure consistent performance and high ligand density. Each batch is tested for key parameters including bead size distribution, exclusion limit, and blue dye content to guarantee reproducibility in chromatographic applications. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in the supplied suspension medium (typically containing 20% ethanol) to prevent microbial growth and maintain bead integrity. Do not freeze. For long-term storage, ensure the container is sealed to prevent evaporation.

Specification

Item	Specification
Matrix	Sepharose 4B, cross-linked
Ligand	Blue Dextran 2000
Bead Size	45-165 μm
Exclusion Limit	> 2 x 106 Daltons
Storage Solution	20% Ethanol
Operating pH Range	3-10 (short term); 4-9 (long term)
Maximum Flow Pressure	0.3 MPa, 3 bar
Ligand Density	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.