Description

Glutamyl-Glycyl-Arginine-4-Nitroanilide is a synthetic tripeptide chromogenic substrate designed for enzymatic activity assays. This compound is critical for biochemical research and diagnostic development, providing a reliable and sensitive means to detect and quantify specific protease activity. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and clinical diagnostics industries for studying enzyme kinetics, inhibitor screening, and assay development.

Application

• Protease Activity Assays: As a chromogenic substrate for the specific detection and kinetic analysis of proteolytic enzymes, particularly those recognizing the glutamyl-glycyl-arginine sequence.
• Biochemical Research: A fundamental tool in academic and industrial laboratories for studying enzyme mechanisms, substrate specificity, and protein-protein interactions.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors for drug discovery programs.
• Diagnostic Kit Manufacturing: Incorporated as a key reagent in the formulation of clinical diagnostic kits for measuring specific enzyme levels in biological samples.
• Quality Control in Bioprocessing: Employed to monitor and ensure the activity and consistency of enzyme batches used in biomanufacturing processes.

Basic Information

Product Name	Glutamyl-Glycyl-Arginine-4-Nitroanilide
CAS No.	67615-71-8
Molecular Formula	C20H30N10O7
Molecular Weight	522.52 g/mol
Synonyms	Glu-Gly-Arg-pNA; L-Glutamyl-glycyl-L-arginine 4-nitroanilide; N-(N-L-Glutamylglycyl)-L-arginine 4-nitroanilide; 4-Nitroanilide of Glu-Gly-Arg; Chromogenic substrate Glu-Gly-Arg-pNA; Protease substrate 67615-71-8; p-Nitroanilide derivative of Glu-Gly-Arg
EINECS	Contact for details

Quality Control

Our Glutamyl-Glycyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and moisture content analysis, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Extinction Coefficient	~9,900 M-1cm-1 at 405 nm (in water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.