Description

4-Hydroxycyclophosphamide is a critical intermediate metabolite of the widely used chemotherapeutic agent cyclophosphamide. This compound matters significantly in pharmaceutical research and development, particularly for studying the metabolic activation, pharmacokinetics, and mechanism of action of alkylating anticancer drugs. Researchers and manufacturers in the global pharmaceutical and biotechnology sectors require high-purity 4-Hydroxycyclophosphamide CAS NO 67292-62-0 for advanced oncology studies, drug metabolism investigations, and as a reference standard.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for quality control and analytical method development in drug manufacturing.
• Oncology Research: Essential for in vitro and in vivo studies investigating the metabolic pathway, efficacy, and toxicity profile of cyclophosphamide and related prodrugs.
• Drug Metabolism and Pharmacokinetics (DMPK): A key analyte for studying the bioactivation and disposition of oxazaphosphorine agents in preclinical and clinical settings.
• Biochemical Assay Development: Serves as a substrate or standard in enzymatic assays to study aldophosphamide metabolism and DNA alkylation mechanisms.
• Toxicology Studies: Employed to assess the specific toxic effects of the active metabolite, separate from the parent drug cyclophosphamide.
• Academic Research: Used in university and institutional labs for fundamental cancer research and pharmacology education.

Basic Information

Product Name	4-Hydroxycyclophosphamide
CAS No.	67292-62-0
Molecular Formula	C7H15Cl2N2O3P
Molecular Weight	277.09 g/mol
Synonyms	4-Hydroxycyclophosphamide; 4-Hydroxy-CPA; 4-OH-CPA; Aldophosphamide; N,N-Bis(2-chloroethyl)-1,3,2-oxazaphosphinan-4-amine 2-oxide; Cyclophosphamide Monohydrate (4-hydroxy metabolite); NSC-26271 (metabolite); 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 4-ol 2-oxide
EINECS	Contact for details

Quality Control

Our 4-Hydroxycyclophosphamide is produced and handled under strict quality management systems suitable for research and reference standard applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure consistency and reliability. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize condensation and exposure. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.