Description

3-(2-Maleimidoethylcarbamoyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is a specialized spin-labeling reagent that combines a stable nitroxide radical with a maleimide functional group. This bifunctional nature is critical for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein dynamics, structure, and conformational changes. It is an essential tool for researchers and developers in structural biology, biophysics, and pharmaceutical sciences who require precise molecular probes for advanced analytical techniques.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Covalently attaches to cysteine residues in proteins to introduce a paramagnetic nitroxide spin label for distance measurements and dynamics studies.
• Protein Structure and Dynamics Analysis: Used to investigate protein folding, conformational changes, oligomerization, and interactions with ligands or other biomolecules.
• Membrane Protein Studies: A valuable probe for analyzing the structure and environment of membrane-embedded proteins due to its stability.
• Drug Discovery and Biophysical Characterization: Employed in pharmaceutical research to characterize target protein-ligand interactions and validate drug binding mechanisms.
• Polymer and Material Science: Can be used as a radical initiator or a functional modifier to introduce stable radicals into synthetic polymers for material property analysis.
• Development of Molecular Sensors: Serves as a building block for creating sensitive probes that report on local environmental changes such as polarity, viscosity, or redox state.

Basic Information

Product Name	3-(2-Maleimidoethylcarbamoyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy
CAS No.	66641-27-8
Molecular Formula	C16H24N4O5
Molecular Weight	352.39 g/mol
Synonyms	3-Maleimido-PROXYL; Maleimido-TEMPO; 3-Maleimido-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; MTSSL (Maleimido-TEMPO Spin Label); 1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-carboxamide, N-(2-maleimidoethyl)-; N-(2-Maleimidoethyl)-3-carbamoyl-2,2,5,5-tetramethylpyrrolidin-1-oxyl; PROXYL maleimide; TEMPO maleimide derivative
EINECS	Contact for details

Quality Control

Our 3-(2-Maleimidoethylcarbamoyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is manufactured under strict quality management systems to ensure high purity and batch-to-batch consistency, critical for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the rigorous standards required for biophysical and biochemical research.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, moisture, or light may affect its stability and radical integrity. For long-term storage, consider storing desiccated under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
EPR Activity	Conforms
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.