Description

Blue-Sepharose is a high-performance affinity chromatography medium designed for the purification of proteins and enzymes. This product is critical for achieving high-purity yields in downstream bioprocessing, offering excellent binding capacity and specificity. It is an essential tool for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable and scalable purification solutions.

Application

• Affinity Chromatography: Primary tool for the purification of dehydrogenases, kinases, and other nucleotide-binding proteins.
• Biopharmaceutical Manufacturing: Downstream purification of recombinant proteins and therapeutic enzymes.
• Enzyme Isolation: Selective capture and purification of specific enzymes from complex biological mixtures.
• Diagnostic Reagent Production: Purification of key protein components used in assay kits and diagnostic tests.
• Academic & Industrial Research: Protein characterization, interaction studies, and small-scale preparative purification.
• Process Development: Scaling up purification protocols from laboratory to pilot and production scale.

Basic Information

Product Name	Blue-Sepharose
CAS No.	66456-82-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cibacron Blue F3G-A Sepharose; Blue Sepharose 6 Fast Flow; Cibacron Blue 3GA-Agarose; Affi-Gel Blue; Reactive Blue 2 Sepharose CL-6B; Blue Dextran-Sepharose; Dye-Ligand Chromatography Medium; Cibacron Blue F3GA-Agarose
EINECS	Contact for details

Quality Control

Our Blue-Sepharose is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance. Each lot is tested for key parameters including ligand density, binding capacity, and flow characteristics. A Certificate of Analysis (COA) detailing these specifications is provided with every shipment to guarantee compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C in 20% ethanol or an appropriate storage buffer as specified. Do not freeze. Keep the suspension homogeneous and avoid drying out the medium.

Specification

Item	Specification
Matrix	Cross-linked 6% agarose beads
Ligand	Cibacron Blue F3G-A
Ligand Density	Approx. 2-8 µmol/mL gel
Binding Capacity	>10 mg albumin/mL gel
Particle Size	45-165 µm
Maximum Flow Rate	300 cm/h
pH Stability	3-13 (long term), 2-14 (short term)
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.