Description

L-Proline-2,5,5-D3 is a stable isotope-labeled analog of the amino acid L-proline, specifically deuterated at the 2, 5, and 5 positions. This high-purity compound is essential for advanced analytical research, providing a critical internal standard for precise quantification and metabolic pathway tracing. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors for applications in mass spectrometry, NMR spectroscopy, and metabolic studies.

Application

• Internal Standard for Mass Spectrometry (MS): Provides accurate quantification of L-proline in complex biological matrices for pharmacokinetic and metabolomic studies.
• Metabolic Pathway Tracing: Used in isotope-labeling experiments to investigate proline metabolism, collagen synthesis, and cellular stress responses.
• NMR Spectroscopy: Serves as a deuterated probe for structural analysis and dynamic studies of proteins and peptides without signal interference.
• Pharmaceutical Research & Development: Critical for the development and validation of bioanalytical methods for drug candidates targeting proline-related pathways.
• Biochemical Research: Employed in enzymatic assays and studies of amino acid transport and utilization.
• Quality Control & Reference Standard: Acts as a certified reference material (CRM) for ensuring accuracy and compliance in analytical laboratories.

Basic Information

Product Name	L-Proline-2,5,5-D3
CAS No.	65807-22-9
Molecular Formula	C₅H₆D₃NO₂
Molecular Weight	118.14 g/mol
Synonyms	(2S)-Pyrrolidine-2-carboxylic-2,5,5-d3 acid; L-Proline-d3; (S)-Proline-2,5,5-d3; Proline-2,5,5-d3; Deuterated L-proline; L-Proline (2,5,5-D3); 2,5,5-Trideutero-L-proline; (S)-2,5,5-Trideuteropyrrolidine-2-carboxylic acid
EINECS	Contact for details

Quality Control

Every batch of L-Proline-2,5,5-D3 is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We guarantee high chemical and isotopic purity, with specifications typically meeting or exceeding the requirements for research and diagnostic use. A detailed Certificate of Analysis (COA) providing results for assay, isotopic enrichment, and impurity profiles is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 99.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Specific Rotation	-84° to -86° (c=1 in H₂O)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.