Description

Percoll is a well-established, ready-to-use density gradient medium for cell separation and purification. Its core value lies in its low viscosity and iso-osmotic formulation, which ensures high cell viability and recovery rates post-separation. This product is essential for researchers and professionals in biotechnology, pharmaceutical development, and clinical diagnostics who require reliable and gentle isolation of viable cells, organelles, or viruses.

Application

• Density Gradient Centrifugation for the isolation of cells, subcellular particles, and viruses.
• Purification of Lymphocytes, Platelets, and other blood cells from whole blood or buffy coat preparations.
• Separation of viable from non-viable cells in cell culture and apoptosis studies.
• Isolation of subcellular organelles such as nuclei, mitochondria, and peroxisomes.
• Virus purification and concentration for vaccine development and virology research.
• Sperm preparation in assisted reproductive technologies (ART).
• Marine biology applications, including the separation of different plankton populations.

Basic Information

Product Name	Percoll
CAS No.	65455-52-9
Molecular Formula	Colloidal silica coated with polyvinylpyrrolidone (PVP)
Molecular Weight	Not applicable (colloidal suspension)
Synonyms	Percoll; Polyvinylpyrrolidone-coated silica; PVP-coated colloidal silica; Density gradient medium; Cell separation medium; Silica, colloidal, PVP-coated
EINECS	Contact for details

Quality Control

Our Percoll is manufactured under strict quality systems. Each batch is tested to ensure consistent performance in density gradient formation, osmolarity, pH, and sterility (where applicable). Certificates of Analysis (COA) detailing batch-specific specifications are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C). Do not freeze. Under these conditions, the product is stable for the duration indicated on the label.

Specification

Item	Specification
Appearance	Clear, colorless to slightly opalescent suspension
Density (at 20°C)	1.130 ± 0.005 g/mL
Osmolality	20 ± 5 mOsm/kg H₂O
pH	8.5 - 9.5 (undiluted)
Viscosity	< 15 cP
Sterility (Sterile Grade)	Sterile (Tested by membrane filtration)
Endotoxin (Cell Culture Grade)	< 0.25 EU/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.