Description

Ethidium Diazide Chloride is a specialized photoactivatable chemical crosslinker derived from the classic nucleic acid stain, ethidium bromide. This compound is critical for researchers requiring precise, light-controlled covalent tethering of biomolecules. It is primarily utilized in advanced molecular biology and biochemistry for applications such as nucleic acid-protein interaction mapping and structural analysis. The product is supplied under stringent quality control to ensure reliability for sensitive experimental workflows.

Application

• Photoaffinity Labeling: Used as a photoactivatable probe to covalently crosslink nucleic acids to interacting proteins or small molecules upon UV irradiation.
• Structural Biology Studies: Employed to "freeze" transient nucleic acid-protein complexes for analysis via techniques like mass spectrometry or electrophoresis.
• Molecular Biology Research: A key tool for identifying and characterizing DNA-binding proteins and mapping their binding sites.
• Bioconjugation: Serves as a versatile intermediate for creating stable conjugates between nucleic acids and other entities for diagnostic or therapeutic development.
• Biochemical Assay Development: Utilized in the design of novel assays that require irreversible capture of biomolecular interactions.

Basic Information

Item	Detail
Product Name	Ethidium Diazide Chloride
CAS No.	65282-36-2
Molecular Formula	C21H20ClN6O2
Molecular Weight	432.88 g/mol
Synonyms	Ethidium Monoazide Chloride; Ethidium Homodiazide Chloride; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Diazide Chloride; EMA Chloride; Photoactivatable Ethidium Bromide Analog; DNA Photo-Crosslinker; Phenanthridinium Diazide Salt
EINECS	Contact for details

Quality Control

Our Ethidium Diazide Chloride is manufactured and tested to meet the exacting standards required for research-grade chemicals. Each batch is subjected to comprehensive analytical characterization to ensure identity, purity, and performance. A Certificate of Analysis (COA) detailing specifications such as purity (HPLC), spectral properties, and functional activity is provided with every shipment to guarantee traceability and consistency for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep container tightly sealed when not in use to prevent degradation.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Photo-crosslinking efficiency confirmed by control assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.