Description

Boc-Leu-Gly-Arg-Amc Acetate Salt is a high-purity, protected peptide sequence featuring a fluorogenic leaving group. This compound is specifically designed as a sensitive and selective substrate for enzymatic assays, particularly for serine proteases. It is an essential research tool for pharmaceutical R&D, diagnostic development, and biochemical studies requiring precise kinetic measurements.

Application

• Protease Activity Assays: Primary substrate for fluorometric determination of trypsin, thrombin, plasmin, and related serine protease activity.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Enzyme Kinetic Studies: Enables precise measurement of kinetic parameters (Km, Vmax, kcat) due to its specific cleavage and fluorescent signal generation.
• Diagnostic Reagent Development: A key component in the formulation of clinical diagnostic kits for coagulation disorders and other protease-related pathologies.
• Biochemical Research: Employed in academic and industrial laboratories to study protease function, regulation, and substrate specificity.
• Quality Control in Biologics: Used to monitor and quantify protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Boc-Leu-Gly-Arg-Amc Acetate Salt
CAS No.	65147-09-3
Molecular Formula	C30H47N9O9 • xC2H4O2
Molecular Weight	677.76 g/mol (free peptide, theoretical)
Synonyms	Boc-Leu-Gly-Arg-AMC acetate; tert-Butyloxycarbonyl-Leucyl-Glycyl-Arginyl-7-Amino-4-Methylcoumarin Acetate; Boc-L-G-R-AMC Acetate; Fluorogenic Substrate for Serine Proteases; Boc-Leu-Gly-Arg-AMC • AcOH; 7-Amino-4-methylcoumarin conjugate of Boc-Leu-Gly-Arg; AMC-labeled peptide substrate; Trypsin substrate Boc-Leu-Gly-Arg-AMC
EINECS	Contact for details

Quality Control

Our Boc-Leu-Gly-Arg-Amc Acetate Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, MS for identity confirmation, and NMR for structural verification. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and impurity profiles to support your regulatory and quality requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep desiccated under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Acetate Content (IC)	1.0 - 3.0 eq
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.