Description

Boc-Val-Pro-Arg-Mca is a fluorogenic peptide substrate designed for the sensitive detection and study of proteolytic enzyme activity. This compound matters significantly in biochemical research as it provides a high-throughput, continuous assay method for enzymes like thrombin and trypsin, where cleavage releases a highly fluorescent group. Researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors need this reagent for enzyme kinetics studies, inhibitor screening, and assay development.

Application

• Protease Activity Assays: Primary substrate for fluorometric determination of serine protease activity, including thrombin and trypsin.
• High-Throughput Screening (HTS): Enables rapid screening of potential enzyme inhibitors or activators in drug discovery pipelines.
• Biochemical Research: Used in kinetic studies to determine enzyme parameters such as Km and Vmax.
• Diagnostic Development: Serves as a key component in developing sensitive clinical assays for coagulation disorders.
• Quality Control: Employed in biopharmaceutical manufacturing to monitor the activity and purity of enzyme-based products.
• Cell Biology Studies: Applied in assays to detect and quantify protease activity in cell lysates and culture media.

Basic Information

Product Name	Boc-Val-Pro-Arg-Mca
CAS No.	65147-04-8
Molecular Formula	C₃₄H₅₀N₈O₉
Molecular Weight	706.81 g/mol
Synonyms	Boc-Val-Pro-Arg-AMC; t-Butyloxycarbonyl-Valyl-Prolyl-Arginyl-4-Methylcoumarin-7-Amide; Boc-V-P-R-MCA; Thrombin Substrate I, Fluorogenic; Boc-Val-Pro-Arg-7-Amido-4-Methylcoumarin; (S)-2-((S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-5-guanidinopentanamido)-N-((S)-1-(((S)-1-((tert-butoxycarbonyl)amino)-3-methylbutyl)amino)-1-oxopropan-2-yl)-5-ureidopentanamide (related IUPAC form); Fluorogenic Thrombin Substrate
EINECS	Contact for details

Quality Control

Our Boc-Val-Pro-Arg-Mca is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.