Description

Coenzyme F420 CAS NO 64885-97-8 is a unique, low-potential, two-electron redox cofactor essential for specific enzymatic reactions in methanogenic and some actinobacterial organisms. Its significance lies in its role as a critical electron carrier in pathways like methanogenesis and tetracycline biosynthesis, making it a valuable tool for biochemical research and enzyme mechanism studies. This compound is primarily needed by research institutions, biotechnology companies, and pharmaceutical developers focusing on anaerobic microbiology, novel antibiotic production, and the study of unique metabolic pathways.

Application

• Biochemical Research: Used as a specialized cofactor in enzymatic assays to study methanogen metabolism and electron transfer mechanisms.
• Enzyme Characterization: Essential for the in vitro activity and study of F420-dependent enzymes, such as dehydrogenases and reductases.
• Antibiotic Biosynthesis Studies: Employed in research on the biosynthesis of natural products like tetracyclines in Streptomyces species.
• Metabolic Pathway Analysis: A key reagent for investigating unique anaerobic and hydroaromatic metabolic pathways in microorganisms.
• Pharmaceutical Development: Serves as a reference standard and research tool in developing drugs that target F420-dependent processes in pathogens like Mycobacterium tuberculosis.
• Educational & Diagnostic Kits: Used in advanced microbiology and biochemistry educational settings and in the formulation of specialized diagnostic enzyme kits.

Basic Information

Product Name	Coenzyme F420
CAS No.	64885-97-8
Molecular Formula	C29H34N5O18P2
Molecular Weight	842.56 g/mol
Synonyms	8-Hydroxy-5-deazaflavin; FO; Factor 420; 5-Deazaflavin; 8-Hydroxy-5-deazariboflavin; Coenzyme FO; F420 Cofactor; 7,8-Didemethyl-8-hydroxy-5-deazariboflavin
EINECS	Contact for details

Quality Control

Our Coenzyme F420 is produced and handled under strict quality protocols to ensure high purity and batch-to-batch consistency for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as purity (HPLC), identity (UV-Vis/IR spectroscopy), and moisture content. We adhere to relevant quality management standards to guarantee the material's integrity and suitability for sensitive biochemical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep containers sealed under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (UV-Vis)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, methanol; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.