Description

3,3',5,5'-Tetramethylbenzidine Dihydrochloride is a high-purity chromogenic substrate widely used in enzymatic assays. Its primary value lies in its high sensitivity and low background signal, making it an essential reagent for precise colorimetric detection. This compound is critical for diagnostic kit manufacturers, research laboratories, and quality control departments in the biotechnology and pharmaceutical industries.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): As a key chromogenic substrate for horseradish peroxidase (HRP) in diagnostic and research immunoassays.
• Clinical Diagnostics: Used in the development and production of test kits for detecting antigens, antibodies, and hormones.
• Immunohistochemistry (IHC): Employed as a staining reagent for visualizing tissue antigens in pathology and biomedical research.
• Western Blotting: Acts as a detection substrate for HRP-conjugated antibodies to visualize protein bands.
• Biosensor Development: Utilized in the fabrication of optical and electrochemical biosensors for analyte detection.
• Research & Development: Serves as a fundamental reagent in biochemical and molecular biology research involving peroxidase enzymes.
• Quality Control Testing: Applied in in-process testing and final product release within biopharmaceutical manufacturing.

Basic Information

Item	Details
Product Name	3,3',5,5'-Tetramethylbenzidine Dihydrochloride
CAS No.	64285-73-0
Molecular Formula	C16H20N2·2HCl
Molecular Weight	317.26 g/mol
Synonyms	TMB Dihydrochloride; 3,3',5,5'-Tetramethylbenzidine 2HCl; TMB-2HCl; TMB dihydrochloride salt; 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride; TMB Substrate; TMB Chromogen; Tetramethylbenzidine Dihydrochloride
EINECS	Contact for details

Quality Control

Our 3,3',5,5'-Tetramethylbenzidine Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, to ensure it meets the high standards required for sensitive diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in DMSO or ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.