Description

p-Tolyltetrazolium Red is a vital tetrazolium salt derivative widely used as a redox indicator and a chromogenic substrate in biochemical assays. Its primary value lies in its ability to produce a measurable formazan dye upon reduction, making it indispensable for quantifying cellular metabolic activity and dehydrogenase enzymes. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Cell Viability and Proliferation Assays: Used in MTT and related assays to measure mitochondrial activity in live cells.
• Microbiological Testing: Acts as an indicator for bacterial growth and metabolic activity in various culture media.
• Enzyme Activity Studies: Serves as a substrate for dehydrogenase enzymes in biochemical research and diagnostic kits.
• Histochemistry and Staining: Employed in tissue staining to localize sites of enzymatic activity.
• Pharmaceutical Quality Control: Utilized in sterility testing and other microbiological quality assurance procedures.
• Toxicology Screening: Applied in in vitro tests to assess the cytotoxic effects of chemical compounds.

Basic Information

Product Name	p-Tolyltetrazolium Red
CAS No.	64225-84-9
Molecular Formula	C19H17N5O
Molecular Weight	331.37 g/mol
Synonyms	p-Tolyltetrazolium Red; 2-(4-Methylphenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; Tetrazolium Red; p-TTC; INT; Iodonitrotetrazolium chloride; 4-Methyl-2-(4-nitrophenyl)-3,5-diphenyl-2H-tetrazolium chloride
EINECS	Contact for details

Quality Control

Our p-Tolyltetrazolium Red is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including identification by spectroscopic methods and purity assessment by HPLC, to ensure it meets high-grade specifications for research and industrial use. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept tightly sealed in a desiccated environment away from direct light exposure to maintain stability and performance.

Specification

Item	Specification
Appearance	Red to dark red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in water, ethanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.