Description

n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Phosphatidylethanolamine is a high-purity, fluorescently labeled phospholipid derivative, essential for advanced membrane biophysics and cell biology research. Its core value lies in serving as a critical fluorescent probe for real-time visualization and quantitative analysis of lipid dynamics, membrane trafficking, and cellular signaling pathways. This compound is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on developing novel assays for lipid metabolism and membrane-associated processes. The product, identified by CAS No. 64205-19-2, is supplied with guaranteed lot-to-lot consistency for reliable experimental results.

Application

• Fluorescent Cell Membrane Labeling: Used as a vital stain for tracking membrane fluidity, fusion, and endocytosis/exocytosis events in live-cell imaging.
• Lipid Raft and Domain Studies: Acts as a probe to investigate the organization and dynamics of lipid microdomains in model and biological membranes.
• Lipoprotein and Vesicle Research: Employed to fluorescently tag lipoproteins, exosomes, or synthetic liposomes for tracing their uptake, distribution, and metabolism.
• Enzyme Activity Assays: Serves as a substrate for phospholipases (e.g., PLC, PLD) in high-throughput screening (HTS) and mechanistic studies, where the NBD fluorophore provides a sensitive readout.
• Apoptosis Detection: Utilized in assays to monitor the externalization of phosphatidylethanolamine (PE), a key marker during early apoptosis.
• Diagnostic Development: Incorporated into diagnostic kits and biosensors for detecting specific lipid-transporting proteins or enzymatic activities related to metabolic disorders.

Basic Information

Product Name	n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Phosphatidylethanolamine
CAS No.	64205-19-2
Molecular Formula	C31H48N4O11P
Molecular Weight	692.70 g/mol
Synonyms	NBD-PE; 1,2-Diacyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl); N-(7-Nitro-2,1,3-benzoxadiazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine; NBD Phosphatidylethanolamine; Fluorescent PE; NBD Fluorescent Lipid; NBD-DHPE; 18:1 NBD PE (common for oleoyl variants)
EINECS	Contact for details

Quality Control

Our n-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Phosphatidylethanolamine is produced under strict quality management protocols to ensure the high purity and performance required for sensitive research applications. Each batch is characterized using advanced analytical techniques including HPLC, mass spectrometry, and NMR to confirm identity and purity. Certificates of Analysis (COA) detailing lot-specific results for purity, fluorescence properties, and solvent content are provided with every shipment. We adhere to relevant standards for research-grade biochemicals to guarantee reliability and reproducibility in your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store solutions under an inert atmosphere such as argon or nitrogen. Allow the product to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 98% (Area %)
Extinction Coefficient (ε)	~21,000 cm-1M-1 at ~460 nm in methanol
Fluorescence Emission	~534 nm (in lipid membranes)
Solvent Residue (GC)	Meet ICH guidelines
Water Content (KF)	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.