Description

Phospholipase C, Phosphatidylinositol-Specific is a highly specific enzyme that catalyzes the hydrolysis of phosphatidylinositol, a key phospholipid in cellular membranes. This precise catalytic action makes it an indispensable tool for research into intracellular signaling pathways, particularly the phosphoinositide cascade. It is essential for biochemists, molecular biologists, and pharmaceutical researchers studying cell communication, membrane dynamics, and drug discovery targets.

Application

• Biochemical Research: Fundamental studies of phosphoinositide-specific phospholipase C (PI-PLC) enzymes and their role in signal transduction.
• Cell Signaling Pathway Analysis: Used to cleave phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3) and diacylglycerol (DAG) in vitro assays.
• Membrane Biology Studies: Investigation of lipid raft composition, membrane protein localization, and the structural role of inositol phospholipids.
• Pharmaceutical Development: Screening and characterization of potential therapeutic agents that modulate PLC-mediated pathways implicated in various diseases.
• Diagnostic Reagent Manufacturing: A component in specialized diagnostic kits for research into G-protein coupled receptor (GPCR) activity and related disorders.
• Enzyme Characterization: Serves as a standard or reference material for comparing the activity and specificity of other phospholipases.

Basic Information

Product Name	Phospholipase C, Phosphatidylinositol-Specific
CAS No.	63551-76-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Phosphatidylinositol Phosphodiesterase; 1-Phosphatidylinositol Phosphodiesterase; PI-PLC; Phosphoinositide Phospholipase C; Inositol Phospholipid-Specific Phospholipase C; Phosphatidylinositol-Specific Phospholipase C; PLC; Phospholipase C (Bacterial); Clostridium perfringens Alpha Toxin (PLC component); Lecithinase C (specific to inositol lipids)
EINECS	Contact for details

Quality Control

Our Phospholipase C, Phosphatidylinositol-Specific is produced and tested under stringent conditions suitable for research applications. Each lot undergoes rigorous quality testing to ensure high specific activity, purity, and functional consistency. Certificates of Analysis (COA) detailing assay results, purity, and activity units are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Hydrolyzes phosphatidylinositol specifically
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content	By Bradford or similar method
Solubility	Clear solution in recommended buffer
Stability	Retains ≥ 90% activity after 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.