Description

10-N(2)-Deoxyguanosine-3-Nitrobenzo(A)Pyrene-7,8,9-Triol is a complex and highly specific nucleoside adduct formed from the covalent binding of a nitro-polycyclic aromatic hydrocarbon metabolite to deoxyguanosine. This compound is of critical importance as a biomarker and reference standard for studying DNA damage and mutagenesis caused by environmental carcinogens. It is primarily utilized by research institutions, diagnostic laboratories, and pharmaceutical companies engaged in toxicology, cancer research, and the development of chemopreventive agents.

Application

• Biomarker Standard for DNA Adduct Analysis: Used as a precise reference material in mass spectrometry (LC-MS/MS) and 32P-postlabeling assays to quantify exposure to nitro-PAHs.
• Mechanistic Toxicology Studies: Essential for investigating the metabolic activation pathways of benzo(a)pyrene derivatives and their specific interactions with DNA.
• Cancer Research: Serves as a tool to study the relationship between specific DNA adduct formation, mutation spectra, and carcinogenic potency.
• Environmental Exposure Assessment: Enables the biomonitoring of human and ecological exposure to combustion-related pollutants.
• Analytical Method Development: Used to validate and calibrate sensitive analytical techniques for detecting ultra-trace levels of DNA damage.
• Standards for Quality Control: Employed in the production and QC of diagnostic kits and assays designed to detect genetic toxicity.

Basic Information

Product Name	10-N(2)-Deoxyguanosine-3-Nitrobenzo(A)Pyrene-7,8,9-Triol
CAS No.	62624-73-1
Molecular Formula	C30H24N6O7
Molecular Weight	556.55 g/mol
Synonyms	3-Nitrobenzo[a]pyrene-7,8,9-triol 10-(2-deoxyguanosin-N2-yl) adduct; N2-(10-[7,8,9-Trihydroxy-7,8,9,10-tetrahydrobenzo[a]pyren-3-yl]-2-deoxyguanosine; 3-Nitro-BPDE-dG Adduct; 10-(Deoxyguanosin-N2-yl)-3-nitro-7,8,9,10-tetrahydrobenzo[a]pyrene-7,8,9-triol; N2-(3-Nitrobenzo[a]pyren-10-yl)-2'-deoxyguanosine; DNA Adduct of 3-Nitrobenzo[a]pyrene
EINECS	Contact for details

Quality Control

Every batch of our 10-N(2)-Deoxyguanosine-3-Nitrobenzo(A)Pyrene-7,8,9-Triol is manufactured and analyzed under strict quality protocols to ensure the identity, purity, and stability required for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing results from HPLC, MS, and NMR characterization. Our quality system is designed to support GLP/GMP-compliant research, and custom purity grades are available to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light will lead to degradation. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light tan solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum conforms to structure
Purity (HPLC)	≥ 95% (Area Percent)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.