Description

Cytochrome p-460 CAS NO 62395-29-3 is a specialized heme-containing metalloprotein with unique spectroscopic and catalytic properties. This compound is critical for researchers and developers in the fields of enzymology and biocatalysis, where it serves as a model system for studying complex electron transfer and oxidation mechanisms. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical R&D departments investigating nitrification pathways, novel enzyme functions, and the development of biosensors.

Application

• Enzymology Research: As a model protein for studying the structure and function of hydroxylamine oxidoreductase (HAO) and related enzymes in the nitrogen cycle.
• Biocatalyst Development: Investigation of its catalytic activity for potential use in specialized oxidation reactions and synthetic biology applications.
• Biochemical Assay Standard: Use as a reference standard or reagent in assays measuring nitrification activity or studying ammonia-oxidizing bacteria.
• Academic & Pharmaceutical R&D: Fundamental research into electron transfer proteins, heme protein engineering, and metabolic pathway analysis.
• Biosensor Prototyping: Exploration of its use as a biological component in sensor devices for environmental monitoring of nitrogen compounds.

Basic Information

Product Name	Cytochrome p-460
CAS No.	62395-29-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytochrome P460; Cytochrome c-460; P460 Cytochrome; HAO-associated cytochrome; Hydroxylamine oxidoreductase cytochrome; Nitrosomonas europaea cytochrome P460; c-type cytochrome P460; Heme P460 protein
EINECS	Contact for details

Quality Control

Our Cytochrome p-460 is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and reliability for research applications. Each lot is characterized for purity and activity using advanced analytical techniques. A comprehensive Certificate of Analysis (COA) detailing spectroscopic properties, purity assessment, and biological activity is provided with every shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive) and light-sensitive; aliquoting is recommended to minimize repeated freeze-thaw cycles and exposure. Handle under controlled, dry conditions to maintain stability and activity.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (UV-Vis)	Characteristic Soret and alpha/beta bands present
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	≥ 85%
Specific Activity	Contact for details
Solubility	Soluble in appropriate aqueous buffers
Endotoxin Level	< 1.0 EU/mg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.