Description

Ac-Ala-Ala-Pro-Ala-Amc is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly elastase and related serine proteases. It is an essential tool for biochemical research, pharmaceutical development, and diagnostic applications where accurate enzyme kinetics are paramount.

Application

• Biochemical Research: Used as a specific fluorogenic substrate for studying the kinetics and inhibition of human leukocyte elastase (HLE) and related proteases.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize novel elastase inhibitors for therapeutic development.
• Diagnostic Development: Serves as a key component in developing in vitro diagnostic tests for conditions involving dysregulated protease activity, such as chronic obstructive pulmonary disease (COPD) and certain inflammatory disorders.
• Enzyme Characterization: Facilitates the determination of specific activity, optimal pH, and temperature profiles for purified protease enzymes in academic and industrial settings.
• Quality Control (QC): Utilized as a reference standard in QC laboratories for validating the activity of enzyme preparations and diagnostic kits.

Basic Information

Product Name	Ac-Ala-Ala-Pro-Ala-Amc
CAS No.	62037-45-0
Molecular Formula	C24H33N7O7
Molecular Weight	531.57 g/mol
Synonyms	N-Acetyl-Ala-Ala-Pro-Ala-7-amido-4-methylcoumarin; Ac-AAPR-AMC; Ac-AAPR-AMC Fluorogenic Substrate; Acetyl-Ala-Ala-Pro-Ala-AMC; Elastase Substrate IV; HLE Substrate; 7-Amino-4-methylcoumarin-labeled peptide substrate; N-Acetyl-L-alanyl-L-alanyl-L-prolyl-L-alanyl-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Pro-Ala-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and NMR for structural validation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment to ensure traceability and consistency for your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in elastase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.