Description

Luciferase is a critical enzyme that catalyzes the light-emitting reaction in bioluminescent organisms, serving as a fundamental tool in molecular and cellular biology. Its primary commercial value lies in its use as a highly sensitive reporter for gene expression, cell viability assays, and high-throughput screening in drug discovery. This product is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors who require reliable and consistent enzymatic activity for their analytical workflows.

Application

• Reporter Gene Assays: Widely used to measure promoter activity and gene expression levels in live cells.
• Bioluminescence Imaging (BLI): Enables non-invasive, real-time monitoring of biological processes in small animal models for preclinical research.
• ATP Detection & Cell Viability Assays: Forms the basis of sensitive assays to quantify cellular ATP, a key indicator of cell health and cytotoxicity.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening compound libraries against therapeutic targets.
• Environmental Monitoring: Used in biosensors to detect specific contaminants or toxicants based on light output.
• Protein-Protein Interaction Studies: Utilized in techniques like the two-hybrid system to study intracellular protein interactions.

Basic Information

Product Name	Luciferase
CAS No.	61970-00-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Firefly Luciferase; Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing); Beetle Luciferase; EC 1.13.12.7; Luciferase (Photinus pyralis); D-Luciferin:oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolyzing)
EINECS	Contact for details

Quality Control

Our Luciferase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is characterized for specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing assay results, including activity units, protein concentration, and purity profile, is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. Aliquot reconstituted enzyme to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	> 1 x 10^7 RLU/µg (typical)
Purity (SDS-PAGE)	> 95%
Protein Concentration	Determined by lot (COA)
Stabilizers / Buffers	Specified per grade (COA)
Endotoxin Level	< 1.0 EU/µg (for cell-based applications)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.