Description

Sp Sephadex is a strong cation exchange chromatography medium, derived from cross-linked dextran beads, widely recognized for its high capacity and resolution in protein purification. Its primary value lies in its ability to separate and purify positively charged biomolecules, such as proteins, peptides, and enzymes, under mild conditions. This makes it an essential tool for researchers and process engineers in the biopharmaceutical, biotechnology, and life sciences sectors, where high-purity biological products are critical.

Application

• Downstream Bioprocessing: Primary capture and intermediate purification of monoclonal antibodies, recombinant proteins, and other therapeutic biologics.
• Enzyme and Peptide Purification: Isolation and purification of cationic enzymes, peptides, and growth factors from complex fermentation broths or cell lysates.
• Polynucleotide Fractionation: Separation of nucleic acids, including tRNA and plasmid DNA, based on charge differences.
• Desalting and Buffer Exchange: Removal of salts or exchange of buffers for processed biological samples in laboratory-scale preparations.
• Analytical Chromatography: Used in high-performance liquid chromatography (HPLC) and fast protein liquid chromatography (FPLC) systems for analytical separations and quality control.
• Vaccine Development: Purification of vaccine components, including viral proteins and antigens, ensuring high purity and safety standards.

Basic Information

Product Name	Sp Sephadex
CAS No.	61840-62-8
Molecular Formula	(C6H10O5)n • SO3H
Molecular Weight	Variable (Polymeric)
Synonyms	Sephadex SP; SP-Sephadex; Sephadex Cation Exchanger; Sulfopropyl Sephadex; Strong Cation Exchanger Sephadex; Dextran Sulfopropyl Ether; SP Sephadex C-25; SP Sephadex C-50
EINECS	Contact for details

Quality Control

Our Sp Sephadex is manufactured under a strict quality management system to ensure batch-to-batch consistency for critical separations. Each lot is tested for key parameters including ion exchange capacity, particle size distribution, flow rate, and purity. Certificates of Analysis (COA) documenting these specifications are available upon request. While not a final drug product, its production considers principles relevant to GMP environments where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect performance. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Ion Exchange Capacity	≥ 2.0 meq/g (Na+ form, dry)
Particle Size (wet)	40-120 μm (Grade dependent)
Exclusion Limit (Globular Proteins)	~30,000 Daltons
pH Stability	2 - 13 (long term); 2 - 10 (working range)
Maximum Operating Pressure	0.3 MPa (3 bar, 44 psi)
Storage Solution	20% Ethanol in water (for preservative)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.