Description

Hemoglobin J Providence CAS NO 61614-15-1 is a specific variant of hemoglobin, a critical oxygen-transporting metalloprotein found in red blood cells. This product is essential for biochemical research and diagnostic development, offering a high-purity reference standard for studying hemoglobinopathies and oxygen-binding kinetics. It is primarily utilized by researchers and manufacturers in the fields of clinical diagnostics, hematology research, and biopharmaceutical development.

Application

• Clinical Diagnostic Reagents: Used as a key component or calibrator in assays for detecting and quantifying abnormal hemoglobin variants.
• Biomedical Research: Serves as a critical research tool for studying protein structure-function relationships, mutation effects, and oxygen transport mechanisms.
• Hematology Standards: Acts as a reference material for quality control and method validation in clinical laboratories.
• Biochemical Studies: Employed in kinetic and thermodynamic studies of ligand binding to hemoproteins.
• Educational Purposes: Used in advanced university and institutional laboratories for teaching protein chemistry and molecular biology.
• Therapeutic Development: Potential use in research for blood substitutes and oxygen-carrying therapeutics.

Basic Information

Product Name	Hemoglobin J Providence
CAS No.	61614-15-1
Molecular Formula	C2952H4664N812O840S8Fe4
Molecular Weight	~64,500 Da (for α2β2 tetramer)
Synonyms	Hb J Providence; Hemoglobin variant J Providence; α-2 globin chain variant; Hbα2 82(E6)Lys→Asn, β2; HBA2 K82N; Hemoglobin alpha chain mutant J Providence; Deoxyhemoglobin J Providence; Oxyhemoglobin J Providence
EINECS	Contact for details

Quality Control

Our Hemoglobin J Providence is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous analytical testing, including purity assessment by electrophoresis and HPLC, confirmation of identity, and verification of functional properties. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is easily oxidized; for optimal preservation, consider storage under an inert atmosphere or as a lyophilized powder. Avoid repeated freeze-thaw cycles. Keep container tightly sealed in a dry environment.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Electrophoresis/HPLC)	Conforms to standard for Hb J Providence
Purity (by HPLC)	≥ 95%
Protein Content (Biuret/BCA)	≥ 90%
Methemoglobin Content	≤ 5%
Oxygen Affinity (P50)	Reported value provided
Solubility	Clear solution in buffer
Endotoxin	< 1.0 EU/mg (if for research use)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.