Description

Imipramine-2,4,6,8-D4 Hcl is a deuterium-labeled analog of the tricyclic antidepressant imipramine hydrochloride, where four hydrogen atoms are replaced with deuterium at the 2, 4, 6, and 8 positions of the dibenzazepine ring system. This stable isotope-labeled compound is essential as an internal standard in quantitative analytical methods, ensuring high accuracy and precision in mass spectrometry-based assays. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in drug metabolism and pharmacokinetics (DMPK) studies, bioanalytical method development, and clinical research.

Application

• Internal Standard for LC-MS/MS: Serves as a critical reference compound in the quantitative bioanalysis of imipramine and its metabolites in biological matrices like plasma, serum, and urine.
• Drug Metabolism Studies (DMPK): Used in pharmacokinetic and pharmacodynamic research to track and quantify the parent drug, enabling precise calculation of key parameters such as clearance and half-life.
• Forensic and Clinical Toxicology: Employed in method development and validation for the accurate screening and confirmation of tricyclic antidepressants in forensic and clinical laboratory settings.
• Pharmaceutical Quality Control: Supports analytical method development for impurity profiling and stability testing of imipramine-based drug formulations.
• Isotope Dilution Mass Spectrometry (IDMS): Acts as a spike in IDMS protocols, a gold-standard technique for achieving high metrological accuracy in measurement science.
• Research Chemical: Utilized in academic and industrial research investigating the biochemical pathways and neurological mechanisms of tricyclic antidepressants.

Basic Information

Product Name	Imipramine-2,4,6,8-D4 Hcl
CAS No.	61361-33-9
Molecular Formula	C19H20D4ClN2 • HCl
Molecular Weight	329.30 g/mol (approx.)
Synonyms	Imipramine-d4 Hydrochloride; 5-(3-Dimethylaminopropyl)-10,11-dihydro-2,4,6,8-tetradeuterio-5H-dibenz[b,f]azepine Hydrochloride; 3-(10,11-Dihydro-2,4,6,8-tetradeuterio-5H-dibenz[b,f]azepin-5-yl)-N,N-dimethylpropan-1-amine Hydrochloride; Imipramine-d4 HCl; Deuterated Imipramine HCl; Imipramine (phenyl-d4) Hydrochloride; Imipramine Internal Standard (D4); Tofranil-d4 HCl
EINECS	Contact for details

Quality Control

Our deuterated standards are produced under strict quality protocols to ensure isotopic purity and chemical integrity, which are critical for reliable analytical results. Each batch of Imipramine-2,4,6,8-D4 Hcl is subjected to rigorous analysis via NMR (for isotopic enrichment verification) and HPLC/MS. We provide comprehensive Certificates of Analysis (COA) detailing assay purity, isotopic purity (atom % D), and impurity profiles. Our quality system supports compliance with the standards required for GLP and ISO 17025 accredited laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccant in the container. Keep the container tightly sealed when not in use to prevent moisture absorption and decomposition.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Assay (HPLC)	≥98.0% (chemical purity)
Isotopic Purity	≥98.0 atom % D (by 1H NMR or MS)
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Single Maximum Impurity (HPLC)	≤0.5%
Total Impurities (HPLC)	≤1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.