Description

n-Succinyl-Ala-Ala-Val p-Nitroanilide is a synthetic chromogenic peptide substrate designed for the specific detection and quantification of proteolytic enzyme activity. This compound is of critical importance in biochemical research and pharmaceutical development, serving as a highly sensitive tool for enzyme kinetics and inhibitor screening. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for studying enzymes like elastase and related serine proteases.

Application

• Enzyme Assay Substrate: Primary chromogenic substrate for the spectrophotometric assay of human leukocyte elastase (HLE) and porcine pancreatic elastase.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel elastase inhibitors for therapeutic development.
• Biochemical Research: Essential for kinetic studies, determining enzyme specificity, inhibition constants (Ki), and catalytic efficiency (kcat/Km).
• Diagnostic Development: Employed in the development of clinical diagnostic kits for measuring elastase activity in biological samples.
• Quality Control: Used in pharmaceutical QC to test the potency and consistency of enzyme-based active pharmaceutical ingredients (APIs).
• Inflammation Research: A key reagent in studies investigating the role of elastase in inflammatory diseases such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and rheumatoid arthritis.

Basic Information

Item	Detail
Product Name	n-Succinyl-Ala-Ala-Val p-Nitroanilide
CAS No.	61043-47-8
Molecular Formula	C₂₂H₃₁N₅O₈
Molecular Weight	493.51 g/mol
Synonyms	Suc-Ala-Ala-Val-pNA; Succinyl-Ala-Ala-Val-p-Nitroanilide; N-Succinyl-L-alanyl-L-alanyl-L-valine 4-nitroanilide; Suc-AAV-pNA; Elastase Substrate III; Elastase Chromogenic Substrate; N-Succinyl-Ala-Ala-Val-pNA; Suc-A-A-V-pNA
EINECS	Contact for details

Quality Control

Our n-Succinyl-Ala-Ala-Val p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional testing in enzyme assays to ensure optimal performance. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to guarantee compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Suitable for elastase assay (Lot-specific data on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.