Description

Fluorescein Isothiocyanate-Dextran (FITC-Dextran) is a vital fluorescent tracer conjugate widely used in biomedical and pharmaceutical research. This compound is essential for studying vascular permeability, membrane integrity, and cellular uptake mechanisms due to its excellent water solubility and stable fluorescence. It is a critical reagent for researchers and manufacturers in the fields of drug delivery system development, pharmacokinetic studies, and diagnostic assay production.

Application

• Vascular Permeability and Blood-Brain Barrier Studies: As a standard tracer to assess endothelial barrier function and integrity in animal models.
• Drug Delivery & Nanocarrier Evaluation: To label and track the distribution, release, and cellular uptake of polymeric nanoparticles and liposomes.
• Cell Biology & Microscopy: For fluorescent labeling of endocytic pathways, phagocytosis, and fluid-phase pinocytosis in live-cell imaging.
• Pharmacokinetics and Biodistribution: To model the in vivo behavior of macromolecules and monitor their clearance rates.
• Diagnostic Kit Development: Used as a fluorescent probe in immunoassays and flow cytometry applications.
• Membrane Filtration Testing: Employed to determine the pore size and integrity of filters and dialysis membranes.

Basic Information

Product Name	Fluorescein Isothiocyanate-Dextran
CAS No.	60842-46-8
Molecular Formula	(C6H10O5)n•C21H11NO5S
Molecular Weight	Varies by dextran chain length (e.g., 4kDa, 10kDa, 40kDa, 70kDa, 150kDa)
Synonyms	FITC-Dextran; Fluorescein 5(6)-Isothiocyanate-Dextran; Dextran-FITC; FITC Labeled Dextran; Fluorescein Isothiocyanate Dextran Conjugate; FD; Dextran, fluorescein isothiocyanate-labeled; Fluorescein thiocarbamoyl dextran
EINECS	Contact for details

Quality Control

Our Fluorescein Isothiocyanate-Dextran is produced under strict quality management protocols. Each batch is characterized for key parameters including degree of labeling (DOL), molecular weight distribution, and fluorescence intensity to ensure batch-to-batch consistency for research reproducibility. A comprehensive Certificate of Analysis (COA) detailing purity, specification, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Orange to brownish-yellow powder or lyophilized solid
Identification (IR)	Conforms to structure
Molecular Weight	As specified (e.g., 4,000; 10,000; 40,000 Da)
Degree of Labeling (DOL)	0.003 - 0.02 mol FITC/mol glucose (typical)
Fluorescence (Ex/Em)	~490 nm / ~520 nm
Solubility	Freely soluble in water and aqueous buffers
Purity (HPLC)	>95% (FITC-Dextran conjugate)
Free FITC	< 1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.