Description

Dtnb-Nhs CAS NO 60129-38-6 is a highly reactive, heterobifunctional crosslinking agent widely used for protein modification and bioconjugation. Its core value lies in its ability to specifically target sulfhydryl groups with its dinitrothiophenyl (DNTB) moiety while offering an amine-reactive N-hydroxysuccinimide (NHS) ester for stable amide bond formation. This compound is essential for researchers and manufacturers in biotechnology, pharmaceutical development, and diagnostic assay production who require precise and efficient protein labeling.

Application

• Protein-Protein Crosslinking: For creating stable conjugates between different proteins or peptides.
• Antibody-Drug Conjugate (ADC) Development: As a linker for attaching cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Enzyme Immobilization: For covalently attaching enzymes to solid supports (e.g., beads, plates, sensors) while preserving activity.
• Fluorescent or Biotin Labeling of Proteins: To introduce tags for detection, purification, or imaging studies.
• Surface Modification: For functionalizing gold nanoparticles or other materials with thiol groups for subsequent biomolecule attachment.
• Structural Biology Studies: To investigate protein structure, dynamics, and interactions through crosslinking mass spectrometry (XL-MS).
• Diagnostic Reagent Manufacturing: In the production of conjugated antibodies and antigens for ELISA and lateral flow assays.

Basic Information

Product Name	Dtnb-Nhs
CAS No.	60129-38-6
Molecular Formula	C15H12N4O8S
Molecular Weight	408.34 g/mol
Synonyms	5,5'-Dithiobis(2-nitrobenzoic acid) N-hydroxysuccinimide ester; DTNB-NHS ester; Ellman's reagent NHS ester; Bis(N-succinimidyl) 3,3'-dithiodipropionate analog; S-(2-Nitro-5-succinimidophenyl) 5-thio-2-nitrobenzoate; NHS-DTNB; 3-Carboxy-4-nitrophenyl disulfide bis(N-succinimide ester)
EINECS	Contact for details

Quality Control

Our Dtnb-Nhs is manufactured under strict quality protocols to ensure batch-to-batch consistency and high reactivity. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance-critical parameters. We adhere to ISO 9001 quality management standards, and our production can be aligned with cGMP requirements for pharmaceutical applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile
Functional Activity	Conforms to reference in standard conjugation test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.