Description

5-Bromo-6-Chloro-3-Indolyl Phosphate p-Toluidine Salt is a specialized chromogenic substrate used in molecular biology and diagnostic applications. This compound is critical for the sensitive detection of alkaline phosphatase activity, enabling precise visualization in techniques such as Western blotting, ELISA, and immunohistochemistry. It is an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and in-vitro diagnostic (IVD) kit production sectors.

Application

• Primary substrate for alkaline phosphatase (AP) in colorimetric detection assays.
• Key component in Western blotting and Northern/Southern blotting for nucleic acid and protein detection.
• Used in enzyme-linked immunosorbent assay (ELISA) platforms for clinical diagnostics.
• Essential reagent in immunohistochemistry (IHC) and in-situ hybridization (ISH) for tissue staining.
• Formulation of in-vitro diagnostic (IVD) kits and research-grade detection systems.
• Used in microparticle-based assays and lateral flow test development.
• Substrate for phosphatase-linked reporter gene assays in cell biology.
• Quality control and manufacturing of biochemical reagents for life science research.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl Phosphate p-Toluidine Salt
CAS No.	6769-80-8
Molecular Formula	C21H19BrClN2O4P
Molecular Weight	517.72 g/mol
Synonyms	BCIP p-Toluidine Salt; 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP; 5-Bromo-6-chloro-3-indolyl phosphate; Magenta-Phos; X-Phosphate; Alkaline Phosphatase Substrate; 3-Indolyl phosphate derivative; BCIP, p-toluidine
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl Phosphate p-Toluidine Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance specifications. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards of the life science and diagnostic industries.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Performance (Enzymatic)	Yields intense, insoluble purple/blue precipitate with AP
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.