Description

Acrylamido Buffer CAS NO 6737-24-2 is a specialized chemical reagent designed for precise pH control in sensitive biochemical and polymer synthesis processes. Its high purity and consistent buffering capacity are critical for ensuring reproducibility and optimal performance in advanced research and manufacturing. This product is essential for industries and laboratories requiring reliable and stable buffering agents for electrophoresis, chromatography, and specialty monomer preparation.

Application

• Polyacrylamide Gel Electrophoresis (PAGE): Used as a key component in buffer systems for the preparation and running of polyacrylamide gels for protein and nucleic acid separation.
• Chromatography: Serves as a mobile phase buffer or column equilibration buffer in various chromatographic techniques, including ion-exchange and size-exclusion chromatography.
• Biochemical Research: Provides a stable pH environment for enzymatic assays, protein purification, and other sensitive biochemical reactions where pH control is paramount.
• Polymer Synthesis: Acts as a buffer in the polymerization of acrylamide and other vinyl monomers to control reaction kinetics and polymer properties.
• Diagnostic Reagent Formulation: Employed in the production of buffers for in-vitro diagnostic kits and clinical laboratory reagents.
• Electroporation & Cell Culture: Used in buffer solutions for electroporation protocols and to maintain physiological pH in certain cell culture media preparations.

Basic Information

Product Name	Acrylamido Buffer
CAS No.	6737-24-2
Molecular Formula	C7H13NO4
Molecular Weight	175.18 g/mol
Synonyms	N-(2-Acetamido)-2-aminoethanesulfonic acid; ACES; N-(2-Acetamido)iminodiacetic acid; N-(Carbamoylmethyl)iminodiacetic acid; 2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid; ACES Buffer; Good's Buffer ACES; (Carbamoylmethyl)iminodiacetic acid
EINECS	229-793-6

Quality Control

Our Acrylamido Buffer is manufactured under a strict quality management system. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for research and industrial applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). Keep away from incompatible materials such as strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	6.7 - 7.3
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
UV Absorbance (260 nm)	≤ 0.05
UV Absorbance (280 nm)	≤ 0.05

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.