Description

5-Bromo-6-Chloro-3-Indolyl Sulfate Potassium Salt Hydrate is a high-purity chromogenic substrate widely used in enzymatic detection systems. This compound is valued for its role as a key reagent in molecular biology and diagnostic applications, where it produces an insoluble, colored precipitate upon enzymatic cleavage. It is primarily utilized by manufacturers and research institutions in the life sciences, diagnostics, and biotechnology sectors for applications such as histochemistry, blotting, and immunoassays.

Application

• Chromogenic Substrate for Phosphatase Enzymes: Primarily used as a substrate for alkaline phosphatase (AP) and other phosphatases in enzyme-linked immunosorbent assays (ELISAs), Western blotting, and immunohistochemistry (IHC) to generate a visible signal.
• Molecular Biology Research: Employed in nucleic acid hybridization techniques, including Southern, Northern, and colony blot assays, for the non-radioactive detection of target sequences.
• Diagnostic Kit Manufacturing: A critical raw material in the production of clinical diagnostic kits for detecting various antigens, antibodies, or nucleic acids.
• Microbiology and Cell Biology: Used in situ to localize enzyme activity within tissues or cells, aiding in pathological studies and cellular function research.
• Quality Control in Bioprocessing: Applied in assays to monitor and quantify the activity of phosphatase enzymes during biopharmaceutical production and purification processes.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl Sulfate Potassium Salt Hydrate
CAS No.	6581-24-4
Molecular Formula	C8H4BrClKNO4S · xH2O
Molecular Weight	Contact for details
Synonyms	BCIP; 5-Bromo-4-chloro-3-indolyl phosphate potassium salt (common misnomer); 5-Bromo-6-chloro-3-indolyl phosphate, potassium salt; BCIP, K salt; 5-Bromo-6-chloro-1H-indol-3-yl potassium sulfate; BCIP sulfate potassium salt; 3-Indolyl phosphate, 5-bromo-6-chloro-, potassium salt
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl Sulfate Potassium Salt Hydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet stringent specifications for chromogenic substrates. A Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMF
Performance Test	Yields intense color development with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.