Description

5-Bromo-4-Chloro-3-Indoxyl Sulfate p-Toluidine Salt is a specialized chromogenic substrate salt widely used in enzymatic detection and histochemical staining. This compound is critical for producing a vivid, insoluble blue precipitate upon enzymatic cleavage, enabling precise localization and quantification of enzyme activity. It is an essential reagent for researchers and manufacturers in the life sciences, diagnostic, and biotechnology sectors requiring high-sensitivity detection systems.

Application

• Histochemical Staining Agent: A key substrate for the detection of β-galactosidase (lacZ) and other hydrolytic enzymes in tissue sections, cell cultures, and microbiological assays.
• Molecular Biology Research: Used in blue-white screening for recombinant clone selection in plasmid vectors containing the lacZ gene.
• Diagnostic Reagent Manufacturing: Incorporated into kits and formulations for clinical diagnostics, particularly for enzyme-linked immunosorbent assays (ELISAs) and blotting techniques.
• Microbiology: Employed in chromogenic media for the identification and differentiation of bacterial colonies based on specific enzyme profiles.
• Neuroscience and Developmental Biology: Utilized as a reporter gene substrate to map gene expression patterns in transgenic organisms.
• Biochemical Research: Serves as a tool for studying enzyme kinetics, inhibition, and localization in various in vitro and in vivo models.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl Sulfate p-Toluidine Salt
CAS No.	6581-23-3
Molecular Formula	C15H13BrClNO5S
Molecular Weight	434.69 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl sulfate p-toluidine salt; X-Sulfate p-Toluidine Salt; BCIP Sulfate p-Toluidine Salt; 5-Bromo-4-chloro-1H-indol-3-yl hydrogen sulfate compound with 4-methylbenzenamine (1:1); 3-Indolyl sulfate, 5-bromo-4-chloro-, p-toluidine salt; BCIP Sulfate Salt; Chromogenic Substrate for β-Galactosidase
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl Sulfate p-Toluidine Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.