Description

5-Bromo-4-Chloro-3-Indolyl Sulfate Potassium Salt is a chromogenic substrate widely used in enzymatic detection systems. This compound is critical for the sensitive and specific visualization of sulfatase enzyme activity in molecular biology and diagnostic applications. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and in-vitro diagnostic (IVD) kit production sectors.

Application

• Histochemistry & Cytochemistry: As a substrate for detecting sulfatase activity in tissue sections and cell cultures, producing an insoluble indigo dye.
• Molecular Biology Assays: Used in blotting techniques (e.g., Western, Southern) for the enzymatic detection of specific proteins or nucleic acids.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic kits for enzyme-linked immunosorbent assays (ELISA) and other colorimetric tests.
• Microbiology Research: For studying bacterial sulfatase enzymes and developing reporter gene systems in genetic engineering.
• Biochemical Research: Enzyme kinetics studies and high-throughput screening (HTS) for drug discovery targeting sulfatase pathways.
• Quality Control (QC) in Pharma: Used in analytical methods to test for the presence of specific enzymatic activities during biopharmaceutical production.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl Sulfate Potassium Salt
CAS No.	6578-07-0
Molecular Formula	C8H4BrClKNO4S
Molecular Weight	364.64 g/mol
Synonyms	BCIP; 5-Bromo-4-chloro-3-indolyl phosphate, disodium salt (common misnomer); X-Phosphate; BCIP Salt; 5-Bromo-4-chloro-1H-indol-3-yl sulfate potassium salt; 3-Indolyl phosphate BCIP; 5-Bromo-4-chloro-3-indoxyl sulfate potassium salt; BCIP, Potassium Salt
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl Sulfate Potassium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is tested to meet exacting specifications for purity, identity, and suitability for enzymatic assays. A comprehensive Certificate of Analysis (COA) detailing purity (typically ≥95% by HPLC), identification (IR), and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMF
Heavy Metals	< 20 ppm
Performance Test	Suitable for enzyme detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.