Description

5-Bromo-4-Chloro-3-Indolyl Phosphate p-Toluidine Salt is a high-purity chromogenic substrate widely used in molecular biology and diagnostic applications. Its primary value lies in its role as a key reagent for the detection of alkaline phosphatase (AP) activity in techniques such as Western blotting, ELISA, and immunohistochemistry. This compound is essential for research and diagnostic laboratories, pharmaceutical quality control, and biotechnology companies requiring sensitive and reliable enzymatic detection.

Application

• Western Blotting (Immunoblotting): Used as a substrate for alkaline phosphatase-conjugated secondary antibodies to visualize protein bands.
• Enzyme-Linked Immunosorbent Assay (ELISA): Employed in colorimetric AP-based ELISA kits for the detection of antigens or antibodies.
• Nucleic Acid Hybridization: Serves as a detection reagent in blotting techniques like Southern and Northern blots when using AP-labeled probes.
• Immunohistochemistry (IHC): Applied in tissue staining protocols to localize specific antigens using AP-conjugated antibodies.
• Pharmaceutical & Diagnostic Manufacturing: A critical raw material in the production of in-vitro diagnostic (IVD) kits and reagents.
• Research & Development: Utilized in biochemical assays within academic, government, and industrial R&D settings to study enzyme kinetics and biomarker detection.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl Phosphate p-Toluidine Salt
CAS No.	6578-06-9
Molecular Formula	C21H19BrClN2O4P
Molecular Weight	501.72 g/mol
Synonyms	BCIP p-Toluidine Salt; 5-Bromo-4-chloro-3-indolyl phosphate toluidine salt; BCIP; X-Phosphate; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate compound with 4-methylbenzenamine (1:1); BCIP, p-toluidine salt; Alkaline Phosphatase Substrate BCIP.
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl Phosphate p-Toluidine Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Functional Test	Suitable for use as an AP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.