Description

2-Iminothiolane is a versatile heterocyclic organic compound, widely recognized as a key reagent for introducing thiol groups into biomolecules. Its primary value lies in its role as a highly effective thiolation and cross-linking agent, enabling the modification of proteins, peptides, and other polymers under mild aqueous conditions. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors, where precise molecular conjugation is critical for drug development, assay creation, and material science.

Application

• Bioconjugation and Protein Modification: Primary reagent for introducing sulfhydryl (-SH) groups onto amino groups of proteins, antibodies, and enzymes for labeling or immobilization.
• Cross-linking Agent: Used to create disulfide bridges between molecules, facilitating the study of protein-protein interactions and the construction of stable macromolecular complexes.
• Diagnostic Kit Development: Critical in the preparation of enzyme conjugates and other labeled biomolecules for immunoassays (ELISA, lateral flow tests) and biosensors.
• Drug Delivery System Fabrication: Employed to functionalize nanoparticles, liposomes, and polymer carriers with targeting ligands or therapeutic payloads.
• Surface Chemistry and Immobilization: Used to thiolate surfaces (e.g., gold, glass) or matrices for the specific attachment of biomolecules in chip-based technologies and affinity chromatography.
• Research Tool in Biochemistry: A standard tool for studying protein structure, function, and folding by selectively modifying lysine residues.

Basic Information

Product Name	2-Iminothiolane
CAS No.	6539-14-6
Molecular Formula	C4H7NS
Molecular Weight	101.17 g/mol
Synonyms	Traut's Reagent; 2-Iminothiolane Hydrochloride (common salt form); 2-IT; 1-Aza-2-thia-5-cyclopentene; 2-Iminotetrahydrothiophene; 4,5-Dihydro-1H-thiopyran-2-imine; 2-Imidothiolane; 2-Thio-1-pyrroline
EINECS	229-459-5

Quality Control

Our 2-Iminothiolane is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive life science applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with relevant GMP and ISO 9001 standards for research and production use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.