Description

Actinomycin D-[3H(G)] CAS NO 6412-01-7 is a tritium-labeled derivative of the potent polypeptide antibiotic Actinomycin D, enabling precise tracking and quantification in biochemical research. This radiolabeled compound is essential for studying nucleic acid interactions, DNA transcription inhibition, and the mechanisms of chemotherapeutic agents. It is a critical tool for researchers in pharmaceutical development, oncology research, and molecular biology who require high-specific-activity tracers for sensitive assay systems.

Application

• Biochemical Research: Used as a high-specific-activity tracer to study the binding of Actinomycin D to DNA and its inhibition of RNA polymerase.
• Oncology & Pharmacology: Employed in drug metabolism and pharmacokinetic (DMPK) studies to track the distribution and cellular uptake of this chemotherapeutic agent.
• Molecular Biology Assays: Facilitates sensitive detection in experiments analyzing transcription inhibition, DNA intercalation, and apoptosis induction pathways.
• Radiochemical Synthesis: Serves as a labeled precursor or intermediate in the synthesis of more complex radiolabeled probes for diagnostic or research purposes.
• Quality Control & Reference Standard: Acts as an internal standard in analytical methods like HPLC or LC-MS for quantifying unlabeled Actinomycin D in pharmaceutical formulations.

Basic Information

Item	Detail
Product Name	Actinomycin D-[3H(G)]
CAS No.	6412-01-7
Molecular Formula	C62H86N12O16 * (3H)
Molecular Weight	~1275.4 g/mol (unlabeled)
Synonyms	Dactinomycin-[3H(G)]; Actinomycin IV-[3H(G)]; Actinomycin C1-[3H(G)]; Meractinomycin-[3H(G)]; NSC-3053-[3H(G)]; Cosmegen-[3H(G)]; (2S)-2-[[(2S,4R,6S)-4-Amino-6-methyl-1,2,3,4,5,6-hexahydro-2,5-dioxobenzo[g]pteridine-7-carbonyl]amino]-N,N'-bis[(6S,9R,10S,13R,18aS)-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-2,3,5,6,9,10,13,14,15,16,17,18-dodecahydro-1H-pyrrolo[2,1-i][1,4,7,10,13]oxatetraazacyclohexadecin-10-yl]-3-methylpentanediamide-[3H(G)]
EINECS	Contact for details

Quality Control

Every batch of Actinomycin D-[3H(G)] is manufactured and analyzed under strict quality systems to ensure radiochemical purity, specific activity, and chemical stability. Quality assurance includes rigorous testing via HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, purity, solvent composition, and analytical chromatograms to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. The product is light-sensitive (store away from light) and solutions should be prepared immediately before use. Store in accordance with all local and national regulations for radioactive materials.

Specification

Item	Specification
Appearance	Yellow to orange solution or solid
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity	≥ 95%
Specific Activity	5-30 Ci/mmol (Contact for exact value)
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol or custom (Specified on COA)
Concentration	1 mCi/mL or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.