Description

L-Methionine p-Nitroanilide is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the study of protease activity, particularly for enzymes that cleave at methionine residues. It is an essential reagent for pharmaceutical R&D, diagnostic kit manufacturing, and academic research in biochemistry and molecular biology. The product is supplied as a high-purity analytical standard to ensure reliable and reproducible experimental results.

Application

• Protease Activity Assays: Primary substrate for the kinetic study and quantification of methionine-specific proteases and aminopeptidases.
• Diagnostic Reagent Manufacturing: Key component in the formulation of diagnostic kits for clinical and research laboratories.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) for drug discovery targeting specific enzymatic pathways.
• Biochemical Research: Essential tool for studying enzyme kinetics, inhibition mechanisms, and substrate specificity in academic and industrial settings.
• Quality Control in Bioprocessing: Employed to monitor and validate the activity of enzyme preparations used in biomanufacturing.

Basic Information

Product Name	L-Methionine p-Nitroanilide
CAS No.	6042-04-2
Molecular Formula	C11H15N3O4S
Molecular Weight	285.32 g/mol
Synonyms	L-Methionine 4-nitroanilide; Methionine p-nitroanilide; L-Met-pNA; N-(4-Nitrophenyl)-L-methioninamide; 4-Nitroanilide of L-methionine; Nα-(4-Nitrobenzoyl)-L-methionine amide; Substrate for Methionine Aminopeptidase
EINECS	Contact for details

Quality Control

Our L-Methionine p-Nitroanilide is manufactured under strict quality control protocols to ensure the highest standards of purity and consistency. Each batch is thoroughly characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to light yellow crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.