Description

Sars Protease Substrate CAS NO 587886-51-9 is a specialized fluorogenic peptide designed for the sensitive detection and kinetic analysis of the SARS coronavirus main protease (3CLpro/Mpro) activity. This compound is a critical tool for researchers developing antiviral therapeutics and diagnostic assays targeting SARS-CoV and related coronaviruses. It is primarily utilized by pharmaceutical R&D teams, virology research laboratories, and diagnostic developers engaged in high-throughput screening and mechanistic enzymology studies.

Application

• High-Throughput Screening (HTS): Used as a key reagent in fluorescence-based assays to screen large compound libraries for SARS-CoV 3CLpro/Mpro inhibitors.
• Enzyme Kinetic Studies: Enables precise determination of kinetic parameters (Km, Vmax, kcat) for the SARS coronavirus main protease, essential for understanding viral replication mechanisms.
• Antiviral Drug Development: Serves as a standard substrate to evaluate the potency and efficacy of novel protease inhibitor candidates in vitro.
• Diagnostic Assay Development: Employed in the research and development of enzymatic diagnostic tests for coronavirus detection and characterization.
• Biochemical Research: Facilitates fundamental studies on coronavirus protease specificity, substrate recognition, and catalytic function.
• Quality Control for Inhibitors: Acts as a reference substrate for the quality control and batch-to-batch validation of commercial SARS protease inhibitors.

Basic Information

Product Name	Sars Protease Substrate
CAS No.	587886-51-9
Molecular Formula	C₃₄H₅₀F₃N₉O₁₀
Molecular Weight	777.82 g/mol
Synonyms	SARS-CoV 3CLpro Substrate; SARS-CoV Mpro Fluorogenic Substrate; Dabcyl-KTSAVLQSGFRKME-Edans; 3CL Protease Substrate (SARS-CoV); SARS Coronavirus Main Protease Substrate; Fluorogenic Peptide Substrate for SARS 3C-like Protease; (7-Methoxycoumarin-4-yl)acetyl-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(5-FAM)-NH₂ (common fluorophore variant); Edans-Dabcyl FRET Substrate for SARS Protease
EINECS	Contact for details

Quality Control

Our Sars Protease Substrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation, to ensure consistent performance in sensitive biochemical assays. Certificates of Analysis (COA) detailing purity, identity, and functional activity are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Functional Activity)	≥ 90% (vs. reference standard)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.