Description

4-[3-(4-Iodophenyl)-2-(2,4-Dinitrophenyl)-2H-5-Tetrazolio]-1,3-Benzene Disulfonate is a specialized tetrazolium salt derivative, valued for its role as a critical chromogenic reagent and biochemical indicator in advanced analytical and diagnostic applications. Its unique structure provides high sensitivity and specificity, making it an essential component for precise colorimetric detection and quantification. This compound is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical quality control laboratories requiring reliable and high-purity biochemical reagents.

Application

• Biochemical Assay Reagent: Used as a vital chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other immunoassays for signal generation.
• Cell Viability & Proliferation Testing: Employed in MTT and related assays to measure mitochondrial activity in live cells, a cornerstone of cytotoxicity and drug efficacy studies.
• Diagnostic Kit Manufacturing: Serves as a key component in the production of clinical diagnostic kits for various biomarkers and pathogens.
• Microbiology Research: Applied in studies of microbial metabolism and dehydrogenase activity due to its redox-sensitive properties.
• Pharmaceutical R&D: Utilized in high-throughput screening (HTS) platforms to identify potential therapeutic compounds.
• Histochemistry & Staining: Functions as a staining agent in tissue sections to localize enzymatic activity.

Basic Information

Product Name	4-[3-(4-Iodophenyl)-2-(2,4-Dinitrophenyl)-2H-5-Tetrazolio]-1,3-Benzene Disulfonate
CAS No.	515111-36-1
Molecular Formula	C19H11IN6O10S2
Molecular Weight	698.36 g/mol
Synonyms	WST-5; Water Soluble Tetrazolium Salt 5; 2-(4-Iodophenyl)-3-(2,4-dinitrophenyl)-5-phenyl-2H-tetrazolium-3'-disulfonate; Iodonitrotetrazolium Violet; INT-Violet; 4-[3-(4-Iodophenyl)-2-(2,4-dinitrophenyl)-2H-tetrazol-5-ium-5-yl]benzene-1,3-disulfonate; Tetrazolium Violet; INT Disulfonate Salt
EINECS	Contact for details

Quality Control

Our production of 4-[3-(4-Iodophenyl)-2-(2,4-Dinitrophenyl)-2H-5-Tetrazolio]-1,3-Benzene Disulfonate adheres to stringent quality management protocols to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We maintain compliance with relevant Good Manufacturing Practice (GMP) principles for active pharmaceutical ingredients (APIs) and critical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and away from direct light exposure to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (in Water)	Clear, yellow solution
Biological Activity (Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.