Description

Zinpyr-4 is a high-purity, cell-permeable fluorescent probe specifically designed for the selective detection of zinc ions (Zn²⁺) in biological systems. Its core value lies in its high affinity and selectivity for Zn²⁺ over other biologically relevant metal ions, enabling precise visualization of labile zinc pools in live cells and tissues. This compound is essential for researchers and professionals in neurobiology, molecular biology, and diagnostic assay development who require reliable tools for studying zinc homeostasis, signaling, and its role in various physiological and pathological processes.

Application

• Intracellular Zinc Imaging: Visualization of labile Zn²⁺ in live cells using fluorescence microscopy (e.g., confocal, epifluorescence).
• Neuroscience Research: Studying the role of synaptic zinc in neurotransmission, particularly in glutamatergic neurons.
• Molecular Probe Development: Serving as a key component in the development of biosensors and diagnostic kits for zinc detection.
• Cell Biology Studies: Investigating zinc trafficking, homeostasis, and its signaling functions in various cell types.
• Pharmaceutical Screening: Used in assays to identify compounds that modulate intracellular zinc levels.
• Histochemical Staining: Detection of chelatable zinc in tissue sections for research in metallobiology.

Basic Information

Product Name	Zinpyr-4
CAS No.	502467-23-4
Molecular Formula	C₃₈H₃₈N₆O₄
Molecular Weight	642.75 g/mol
Synonyms	ZP4; Zinpyr-4; 6-[(2,4-Dinitrophenyl)amino]-N,N-bis(2-pyridinylmethyl)-9H-fluoren-2-amine; 6-[(2,4-Dinitrophenyl)amino]-N,N-di-2-pyridinylmethyl-9H-fluoren-2-amine; Fluorescent zinc indicator Zinpyr-4; Zinc Probe 4; Zn²⁺ fluorescent sensor ZP4
EINECS	Contact for details

Quality Control

Our Zinpyr-4 is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, NMR for structural confirmation, and fluorescence spectroscopy for functional validation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and performance characteristics to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Fluorescence Properties	Excitation/Emission maxima consistent with literature
Zinc Binding Affinity (Kd)	≤ 1 nM (in appropriate buffer)
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.