Description

6-Chloro-3-Indoxyl-n-Acetyl-β-D-Galactosaminide is a specialized chromogenic substrate used for the detection of N-acetyl-β-D-galactosaminidase (NAGase) activity. This compound matters for researchers and diagnostic manufacturers as it provides a sensitive and specific colorimetric readout, enabling the visualization and quantification of enzymatic activity in biochemical assays. It is primarily needed by professionals in life science research, clinical diagnostics, and pharmaceutical development for applications in enzyme kinetics, histochemistry, and diagnostic kit formulation.

Application

• Enzyme Activity Assays: Primary substrate for the colorimetric detection and quantification of N-acetyl-β-D-galactosaminidase (NAGase) activity in research and diagnostic settings.
• Diagnostic Kit Development: A key component in the formulation of diagnostic kits for detecting specific bacterial enzymes or genetic markers, such as those from E. coli.
• Microbiology & Cell Biology: Used in chromogenic agar media and staining protocols to differentiate bacterial colonies based on enzyme expression.
• Biochemical Research: Employed in studies of glycosidase enzymes, including inhibitor screening and enzyme characterization.
• Histochemistry & Cytochemistry: Acts as a staining agent for the localization of NAGase activity in tissue sections and cell cultures.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential glycosidase inhibitors for therapeutic development.

Basic Information

Product Name	6-Chloro-3-Indoxyl-n-Acetyl-β-D-Galactosaminide
CAS No.	501432-61-7
Molecular Formula	C16H19ClN2O7
Molecular Weight	386.78 g/mol
Synonyms	6-Chloro-3-indolyl N-acetyl-β-D-galactosaminide; 6-Chloro-3-indoxyl N-acetyl-β-D-galactosaminide; 6-Cl-3-Indoxyl-NAG; NAG-6-Cl; GalNAc-6-Cl-3-Indoxyl; 2-(Acetylamino)-2-deoxy-β-D-galactopyranoside 6-chloro-1H-indol-3-yl; 6-Chloro-3-indolyl 2-acetamido-2-deoxy-β-D-galactopyranoside; Chromogenic NAGase Substrate
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indoxyl-n-Acetyl-β-D-Galactosaminide is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is light-sensitive (store away from light) and may be hygroscopic. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.