Description

p-Nitrophenyl 2-Acetamido-2-Deoxy-3-o-(β-D-Galactopyranosyl)-α-D-Galactopyranoside is a specialized chromogenic substrate designed for the sensitive and specific detection of glycosidase enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise biochemical analysis. It is primarily utilized in the life sciences, pharmaceutical development, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and assay development.

Application

• Enzyme Assay Substrate: A key chromogenic substrate for the detection and quantification of α-N-acetylgalactosaminidase and related glycosidase enzymes.
• Biochemical Research: Used in enzyme kinetics studies to determine kinetic parameters (Km, Vmax) and study enzyme mechanisms.
• Diagnostic Development: Employed in the development of diagnostic kits and assays for genetic disorders related to glycosidase deficiencies, such as Schindler disease.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential therapeutic inhibitors of specific glycosidases.
• Quality Control: Applied in biomanufacturing and pharmaceutical QC labs to monitor enzyme purity and activity during production processes.
• Glycobiology Studies: Essential for research into carbohydrate-active enzymes, glycoprotein processing, and cellular glycosylation pathways.

Basic Information

Product Name	p-Nitrophenyl 2-Acetamido-2-Deoxy-3-o-(β-D-Galactopyranosyl)-α-D-Galactopyranoside
CAS No.	59837-14-8
Molecular Formula	C22H30N2O13
Molecular Weight	530.48 g/mol
Synonyms	pNP-α-D-GalNAc-(1→3)-α-D-Gal; p-Nitrophenyl α-D-GalNAc-(1→3)-α-D-Galactopyranoside; 4-Nitrophenyl 2-acetamido-2-deoxy-3-O-β-D-galactopyranosyl-α-D-galactopyranoside; α-D-GalNAc-(1→3)-α-D-Gal-pNP; PNP-α-D-GalNAc-(1→3)-α-D-Gal; Substrate for α-N-acetylgalactosaminidase; Chromogenic N-acetyl-α-D-galactosaminide substrate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 2-Acetamido-2-Deoxy-3-o-(β-D-Galactopyranosyl)-α-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.