Description

L-Glutamine-Amide-15N is a stable isotope-labeled analog of L-glutamine, where the amide nitrogen is enriched with the 15N isotope. This compound is essential for advanced research in metabolism, protein synthesis, and cellular signaling using techniques like Nuclear Magnetic Resonance (NMR) spectroscopy and Mass Spectrometry (MS). It is a critical tool for scientists and manufacturers in the pharmaceutical, biotechnology, and life science research sectors who require precise isotopic tracers for metabolic flux analysis and drug development studies.

Application

• Metabolic Research & Flux Analysis: Used as a 15N-labeled tracer to study glutamine metabolism, nitrogen assimilation, and amino acid pathways in cells and organisms.
• Biopharmaceutical Development: Employed in the research and production of labeled proteins, peptides, and antibodies for structural and functional studies.
• NMR Spectroscopy: Serves as a crucial standard and substrate in nuclear magnetic resonance studies to elucidate molecular structures and dynamics.
• Mass Spectrometry (MS): Used as an internal standard for the accurate quantification of glutamine and related metabolites in complex biological samples via LC-MS or GC-MS.
• Nutritional Science Research: Applied in studies investigating the role of glutamine in cell culture media, sports nutrition, and clinical nutrition.
• Diagnostic Reagent Manufacturing: Incorporated into the formulation of specialized kits and reagents for clinical diagnostics and research assays.

Basic Information

Item	Details
Product Name	L-Glutamine-Amide-15N
CAS No.	59681-32-2
Molecular Formula	C₅H₁₀¹⁵N₂O₃
Molecular Weight	147.10 g/mol (for ¹⁵N labeled amide)
Synonyms	(2S)-2-Amino-4-carbamoylbutanoic acid-amide-15N; L-Glutamine (amide-15N); Glutamine-amide-15N; (S)-2,5-Diamino-5-oxopentanoic acid-amide-15N; Levoglutamide-amide-15N; 15N-L-Glutamine (amide labeled); 15N-Gln; ¹⁵N-Glutamine
EINECS	Contact for details

Quality Control

Our L-Glutamine-Amide-15N is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high chemical purity and isotopic enrichment. Certificates of Analysis (COA) are provided, detailing parameters such as assay (HPLC), isotopic purity (NMR/MS), optical rotation, and limits for residual solvents and impurities. We adhere to relevant standards for research-grade biochemicals, and custom purity grades are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Enrichment (¹⁵N)	≥ 98 atom % ¹⁵N (amide nitrogen)
Optical Rotation	[α]²⁰/D: +6.0° to +8.0° (c=2 in H₂O)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.