Description

1'-Hydroxymidazolam is a key pharmacologically active metabolite of the sedative-hypnotic drug midazolam. This compound is of significant importance for pharmaceutical research and development, particularly in the study of drug metabolism, pharmacokinetics, and the development of analytical reference standards. It is primarily utilized by research institutions, pharmaceutical companies, and contract testing laboratories engaged in bioanalytical method development, forensic toxicology, and clinical studies related to benzodiazepine pharmacology.

Application

• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for the quantitative and qualitative analysis of midazolam and its metabolites in biological matrices.
• Drug Metabolism & Pharmacokinetics (DMPK) Studies: Used in in vitro and in vivo studies to understand the metabolic pathways and clearance mechanisms of midazolam.
• Forensic & Clinical Toxicology: Essential for developing and validating analytical methods (e.g., LC-MS/MS, GC-MS) to detect and quantify benzodiazepine use in forensic and clinical settings.
• Bioanalytical Method Development: A critical component for calibrating instruments and ensuring accuracy in assays designed to measure drug concentrations in plasma, serum, or urine.
• Pharmacological Research: Investigated for its own potential activity and role in the overall effects and duration of action of its parent drug, midazolam.
• Impurity Profiling: Used as a known impurity or degradation product standard in the quality control of midazolam active pharmaceutical ingredient (API) and finished dosage forms.

Basic Information

Product Name	1'-Hydroxymidazolam
CAS No.	59468-90-5
Molecular Formula	C18H13ClFN3O
Molecular Weight	341.77 g/mol
Synonyms	1'-Hydroxy Midazolam; 1-Hydroxymidazolam; 8-Chloro-6-(2-fluorophenyl)-1-methyl-4H-imidazo[1,5-a][1,4]benzodiazepin-4-ol; α-Hydroxymidazolam; Midazolam Metabolite; 1'-OH-Midazolam; Ro 21-3982/001
EINECS	Contact for details

Quality Control

Our 1'-Hydroxymidazolam is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods (IR, NMR, MS), purity assessment by HPLC, and determination of residual solvents and impurities to ensure it meets high-purity standards suitable for research and analytical applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming batch-specific results for identity, purity, and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Individual impurity: ≤ 0.5% Total impurities: ≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.