Description

Z-Phe-Arg-Pna is a chromogenic peptide substrate widely used in biochemical research and diagnostic applications. This compound is specifically designed for the sensitive and selective detection of serine proteases, particularly those involved in critical physiological pathways. It is an essential reagent for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors requiring precise enzyme activity analysis.

Application

• Protease Activity Assays: Primary substrate for the kinetic measurement of trypsin, thrombin, plasmin, and other related serine proteases.
• Diagnostic Kit Development: Key component in chromogenic test kits for clinical diagnostics, including coagulation factor analysis and inflammatory marker detection.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: Essential tool for studying enzyme kinetics, inhibition mechanisms, and protease function in various biological systems.
• Quality Control in Biologics: Employed in purity and potency testing of enzyme-based pharmaceuticals and recombinant proteins.

Basic Information

Product Name	Z-Phe-Arg-Pna
CAS No.	59188-54-4
Molecular Formula	C30H34N6O6
Molecular Weight	574.63 g/mol
Synonyms	Benzyloxycarbonyl-Phenylalanyl-Arginine 4-Nitroanilide; Z-Phe-Arg-pNA; Z-F-R-pNA; Chromogenic Substrate S-2765 (component); Nα-CBZ-L-Phenylalanyl-L-Arginine 4-Nitroanilide; Boc-Phe-Arg-pNA (related); Protease Substrate; Thrombin Substrate I
EINECS	Contact for details

Quality Control

Our Z-Phe-Arg-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Extinction Coefficient	~8800 M-1cm-1 at 405 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.