Description

Ribonuclease-S-Protein Grade 12-Pr is a high-purity, proteolytically cleaved derivative of bovine pancreatic ribonuclease A, specifically designed for advanced biochemical research and diagnostic applications. This enzyme is critical for the selective digestion of RNA in complex biological samples, enabling precise molecular analysis. It is an essential reagent for life science researchers, pharmaceutical developers, and diagnostic kit manufacturers requiring controlled and specific RNA removal to ensure assay accuracy and purity.

Application

• Molecular Biology Research: Selective RNA digestion in nucleic acid purification protocols and sample preparation for downstream applications like PCR, sequencing, and cloning.
• Pharmaceutical Development: Used in process development for the purification of DNA-based therapeutics (e.g., plasmids, mRNA) by removing contaminating RNA.
• Diagnostic Assay Manufacturing: A key component in diagnostic kits (e.g., for viral RNA detection) to eliminate background RNA interference and improve test specificity.
• Structural Biology Studies: The S-protein fragment is widely used as a model system in protein folding, enzyme kinetics, and protein-ligand interaction studies.
• Cell Culture & Virology: Application in cell lysate preparation to degrade RNA without affecting DNA or proteins, facilitating specific analyte isolation.
• Biochemical Reagent Production: Serves as a high-quality standard or active ingredient in the formulation of enzymatic reagent mixes for research and industrial use.

Basic Information

Product Name	Ribonuclease-S-Protein Grade 12-Pr
CAS No.	59113-63-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	RNase S-Protein; Ribonuclease A S-Protein; RNase A S-Protein; Ribonuclease S Protein Fragment (1-124); Pancreatic Ribonuclease S-Protein; EC 3.1.27.5 (S-Protein); RNase-SP
EINECS	Contact for details

Quality Control

Our Ribonuclease-S-Protein Grade 12-Pr is manufactured under strict quality management systems. Each batch is subjected to rigorous analytical testing to ensure it meets the high-purity standards required for sensitive research and in-vitro diagnostic use. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and absence of specific contaminants (e.g., DNase, protease) is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Purity (SDS-PAGE)	≥ 95%
Specific Activity	≥ 50 Kunitz units/mg protein
Protein Content (Biuret)	≥ 90%
DNase Activity	Not detectable
Protease Activity	Not detectable
Solubility	Soluble in water or buffer (e.g., 10 mM sodium acetate, pH 5.2)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.