Description

Kanamycin Phosphotransferase II is a specialized enzyme crucial for conferring kanamycin resistance in molecular biology and genetic engineering applications. This product is essential for researchers and manufacturers requiring precise selection and maintenance of genetically modified organisms. It is primarily utilized in the biotechnology, pharmaceutical, and life science research sectors for vector construction, protein expression studies, and antibiotic resistance marker validation.

Application

• Molecular Cloning & Vector Construction: Used as a selective marker in plasmid vectors for bacterial transformation.
• Genetic Engineering: Essential for creating and maintaining kanamycin-resistant strains of bacteria, yeast, and other microorganisms.
• Protein Expression Systems: Employed in research and industrial fermentation to ensure plasmid stability in host cells during recombinant protein production.
• Antibiotic Resistance Studies: Serves as a key reagent in biochemical research to study enzyme kinetics and resistance mechanisms.
• Biotechnology Research: A critical tool in academic and industrial labs for genetic manipulation and synthetic biology projects.
• Diagnostic Kit Development: Used in the formulation of kits for detecting specific genetic markers or antibiotic resistance genes.

Basic Information

Product Name	Kanamycin Phosphotransferase II
CAS No.	58943-39-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Kanamycin Kinase II; Kanamycin 3'-Phosphotransferase II; APH(3')-II; Aminoglycoside 3'-Phosphotransferase II; Kanamycin Resistance Protein; Neo/Kanamycin Phosphotransferase II; KANr Protein; Tn5 Kanamycin Resistance Determinant
EINECS	Contact for details

Quality Control

Our Kanamycin Phosphotransferase II is produced and tested under stringent quality management systems to ensure high purity, specific activity, and batch-to-batch consistency suitable for critical research and production applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, activity units, and absence of contaminating nucleases. We adhere to relevant guidelines for enzyme production to support your compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The product is supplied in a suitable buffer to maintain enzymatic activity.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ XX U/mg (Contact for details)
Protein Concentration	XX mg/mL (Contact for details)
Buffer	Tris-HCl, Glycerol, DTT
pH	7.5 ± 0.5
Endotoxin Level	< 1.0 EU/μg (if applicable)
DNase/RNase Activity	Not detected
Identification (Functional Assay)	Confers kanamycin resistance in control E. coli strain

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.