Description

Ethidium Monoazide Bromide is a vital photo-reactive nucleic acid dye used for the selective detection of viable cells in molecular biology. Its core value lies in its ability to penetrate compromised cell membranes and covalently bind to DNA upon photoactivation, making it an essential tool for eliminating false-positive signals from dead cells in PCR and other amplification techniques. This product is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for applications in microbiological safety testing, environmental monitoring, and food pathogen detection. Ethidium Monoazide Bromide CAS NO 58880-05-0 ensures accurate viability assessment in complex samples.

Application

• Viability PCR (vPCR): Selective amplification of DNA from live bacterial, fungal, or mammalian cells by inhibiting DNA from membrane-compromised dead cells.
• Microbiological Quality Control: Accurate detection of viable pathogens in pharmaceutical products, water, and raw materials for sterility testing.
• Food Safety Testing: Differentiation between live and dead foodborne pathogens (e.g., *Salmonella*, *Listeria*, *E. coli*) to assess true contamination risk.
• Environmental Monitoring: Analysis of viable microbial populations in wastewater, soil, and air samples without culture bias.
• Clinical Diagnostics: Improved accuracy in molecular assays for infectious diseases by reducing background from non-viable organisms.
• Biopharmaceutical Process Monitoring: Assessing cell viability and microbial contamination in fermentation and cell culture processes.
• Research on Bacterial Physiology: Studying the effects of antibiotics, disinfectants, and environmental stresses on bacterial viability.

Basic Information

Item	Detail
Product Name	Ethidium Monoazide Bromide
CAS No.	58880-05-0
Molecular Formula	C21H20BrN6+ (commonly listed as the bromide salt)
Molecular Weight	Approx. 452.33 g/mol (for the monobromide cation)
Synonyms	EMA Bromide; Ethidium Homodimer Monoazide Bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium, azide derivative; EMA; Viability Dye EMA; EthD-1 Monoazide; Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, azide, bromide; Propidium Monoazide analog (for viability PCR)
EINECS	Contact for details

Quality Control

Our Ethidium Monoazide Bromide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent photo-reactivity, and performance in viability PCR applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as identity, purity, and functional activity. We support compliance with relevant quality frameworks for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, consider storage under desiccant or inert atmosphere. Keep the container sealed when not in use to prevent moisture absorption and decomposition.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Functional Activity (vPCR)	Effectively inhibits DNA amplification from heat-killed cells
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.